HIGH-AFFINITY FOLATE BINDING IN HUMAN LIVER MEMBRANES

被引：6

作者：

HOLM, J

HANSEN, SI

HOIERMADSEN, M

机构：

[1] CENT HOSP HILLEROD,DEPT CLIN CHEM,DK-3400 HILLEROD,DENMARK

[2] STATE SERUM INST,AUTOIMMUNE SEROL LAB,DK-2300 COPENHAGEN S,DENMARK

来源：

BIOSCIENCE REPORTS | 1991年 / 11卷 / 03期

关键词：

HUMAN LIVER; MEMBRANES; HIGH AFFINITY FOLATE BINDING PROTEINS;

D O I：

10.1007/BF01182482

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-affinity binding of H-3-folate in Triton X-100 solubilized membranes of human liver displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. Ultrogel(R) AcA 44 chromatography of solubilized membranes saturated with H-3-folate revealed a major peak of 100 kDa and a minor peak of 25 kDa. The 100 kDa peak could represent a hydrophobic membrane associated molecular form of the protein. This notion was supported by the fact that the two peaks had identical molecular weights as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis with immunoblotting.

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收藏

页码：139 / 145

页数：7

相关论文

共 16 条

[1] EVIDENCE FOR A FOLIC-ACID BINDING-PROTEIN IN HUMAN CELL-MEMBRANE [J].

CORROCHER, R ;

PACHOR, ML ;

BAMBARA, LM ;

DESANDRE, G .

ACTA HAEMATOLOGICA, 1981, 66 (03) :202-209

[2] CHARACTERIZATION OF THE FOLATE-BINDING PROTEINS ASSOCIATED WITH THE PLASMA-MEMBRANE OF RAT-LIVER [J].

DACOSTA, M ;

ROTHENBERG, SP .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 939 (03) :533-541

[3]

HANSEN S I, 1990, European Journal of Clinical Investigation, V20, pA32

[4] A HIGH-AFFINITY FOLATE BINDING-PROTEIN IN HUMAN-URINE - RADIOLIGAND BINDING CHARACTERISTICS, IMMUNOLOGICAL PROPERTIES AND MOLECULAR-SIZE [J].

HANSEN, SI ;

HOLM, J ;

HOIERMADSEN, M .

BIOSCIENCE REPORTS, 1989, 9 (01) :93-97

[5] COOPERATIVE BINDING OF FOLATE TO A PROTEIN ISOLATED FROM COWS WHEY [J].

HANSEN, SI ;

HOLM, J ;

LYNGBYE, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 535 (02) :309-318

[6] DEPENDENCE OF AGGREGATION AND LIGAND AFFINITY ON THE CONCENTRATION OF THE FOLATE-BINDING PROTEIN FROM COWS MILK [J].

HANSEN, SI ;

HOLM, J ;

LYNGBYE, J ;

PEDERSEN, TG ;

SVENDSEN, I .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 226 (02) :636-642

[7]

HENDERSON GB, 1990, ANNU REV NUTR, V10, P319, DOI [10.1146/annurev.nutr.10.1.319, 10.1146/annurev.nu.10.070190.001535]

[8] RABBIT ANTIBODIES AGAINST THE LOW-MOLECULAR-WEIGHT FOLATE BINDING-PROTEIN FROM HUMAN-MILK - USE FOR IMMUNOLOGICAL CHARACTERIZATION OF HUMAN FOLATE BINDING-PROTEINS IN AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) [J].

HOIERMADSEN, M ;

HANSEN, SI ;

HOLM, J .

BIOSCIENCE REPORTS, 1987, 7 (07) :553-557

[9] A HIGH-AFFINITY FOLATE BINDING-PROTEIN IN NORMAL HUMAN-LEUKOCYTES - LIGAND-BINDING CHARACTERISTICS, IONIC CHARGE AND MOLECULAR-SIZE [J].

HOLM, J ;

HANSEN, SI ;

LYNGBYE, J .

BIOSCIENCE REPORTS, 1985, 5 (08) :683-688

[10] HIGH AND LOW AFFINITY BINDING OF FOLATE TO PROTEINS IN SERUM OF PREGNANT-WOMEN [J].

HOLM, J ;

HANSEN, SI ;

LYNGBYE, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 629 (03) :539-545