PHENYLMETHYLSULFONYL FLUORIDE PROTECTS L-LYSINE TRANSPORT IN SCHIZOSACCHAROMYCES-POMBE AGAINST INACTIVATION BY AMMONIUM-IONS

被引:5
作者
HORAK, J
SYCHROVA, H
KOTYK, A
机构
[1] Department of Membrane Transport, Institute of Physiology, Czechoslovak Academy of Sciences
关键词
(S pombe); (Yeast); Amino acid transport; Ammonium ion; l-Lysine transport;
D O I
10.1016/0005-2736(90)90129-C
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ammonium ions inactivate the basic amino acid transport system in Schizosaccharomyces pombe in an irreversible manner. The inactivation is accompanied by a 4-fold decrease of KT of l-lysine transport, leaving its Jmax unchanged; phenylmethylsulfonyl fluoride protects the system against inactivation. In contrast, two basic amino acid transport systems in a gap1 mutant of Saccharomyces cerevisiae are influenced by NH4 + ions in such a way that only the Jmax decreases while the KT of l-lysine transport is unchanged. Phenylmethylsulfonyl fluoride does not act here as a protective agent. © 1990.
引用
收藏
页码:380 / 382
页数:3
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