PHENYLMETHYLSULFONYL FLUORIDE PROTECTS L-LYSINE TRANSPORT IN SCHIZOSACCHAROMYCES-POMBE AGAINST INACTIVATION BY AMMONIUM-IONS

被引：5

作者：

HORAK, J

SYCHROVA, H

KOTYK, A

机构：

[1] Department of Membrane Transport, Institute of Physiology, Czechoslovak Academy of Sciences

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1023卷 / 03期

关键词：

(S pombe); (Yeast); Amino acid transport; Ammonium ion; l-Lysine transport;

D O I：

10.1016/0005-2736(90)90129-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ammonium ions inactivate the basic amino acid transport system in Schizosaccharomyces pombe in an irreversible manner. The inactivation is accompanied by a 4-fold decrease of KT of l-lysine transport, leaving its Jmax unchanged; phenylmethylsulfonyl fluoride protects the system against inactivation. In contrast, two basic amino acid transport systems in a gap1 mutant of Saccharomyces cerevisiae are influenced by NH4 + ions in such a way that only the Jmax decreases while the KT of l-lysine transport is unchanged. Phenylmethylsulfonyl fluoride does not act here as a protective agent. © 1990.

引用

页码：380 / 382

页数：3

共 10 条

[1]

COOPER TG, 1982, MOL BIOL YEAST SACCH, V2, P399

[2] MULTIPLICITY OF AMINO ACID PERMEASES IN SACCHAROMYCES CEREVISIAE .I. EVIDENCE FOR A SPECIFIC ARGININE-TRANSPORTING SYSTEM [J].