CHARACTERIZATION OF TRYPANOSOMA-CRUZI ISOLATES FROM PARAGUAY, USING RESTRICTION ENZYME ANALYSIS OF KINETOPLAST DNA

被引：7

作者：

MIMORI, T

MALDONADO, M

SAMUDIO, M

DEARIAS, AR

MORENO, R

SAKAMOTO, M

机构：

[1] UNIV NACL ASUNCION, INST INVEST CIENCIAS SALUD, ASUNCION, PARAGUAY

[2] NAGASAKI UNIV, INST TROP MED, DEPT PARASITOL, NAGASAKI 852, JAPAN

来源：

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY | 1992年 / 86卷 / 03期

关键词：

D O I：

10.1080/00034983.1992.11812659

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Eleven Paraguayan strains of Trypanosoma cruzi, from Chagas' disease patients and the bug vectors, were examined by restriction endonuclease analysis of kinetoplast DNA using Hae III, Map I, Eco RI, Hinf I, Taq I and Rsa I. Four schizodeme-profile groups were identified. Group 1 had much simpler profiles than groups 2, 3 and 4 and, although there were homogeneous profiles in the latter three groups, each group could be distinguished from the others. The profiles of group 1 could not be matched with any of the standard strains from Brazil, Chile and Colombia included in the schizodeme comparison. The profiles of groups 3 and 4 shared most features with those standards of the Brazilian Z2 zymodeme.

引用

页码：231 / 237

页数：7

共 25 条

[1] SCHIZODEME ANALYSIS OF TRYPANOSOMA-CRUZI STOCKS FROM SOUTH AND CENTRAL-AMERICA BY ANALYSIS OF PCR-AMPLIFIED MINICIRCLE VARIABLE REGION SEQUENCES [J].

AVILA, H ;

GONCALVES, AM ;

NEHME, NS ;

MOREL, CM ;

SIMPSON, L .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 42 (02) :175-187

[2] TRYPANOSOMA-CRUZI SAMPLES ISOLATION BY XENODIAGNOSIS AND HEMOCULTURE FROM PATIENTS WITH CHRONIC CHAGAS-DISEASE [J].

BRONFEN, E ;

ROCHA, FSD ;

MACHADO, GBN ;

PERILLO, MM ;

ROMANHA, AJ ;

CHIARI, E .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1989, 84 (02) :237-240

[3] ELECTROPHORETIC ANALYSIS OF ENDONUCLEASE-GENERATED FRAGMENTS OF K-DNA, OF ESTERASE ISOENZYMES, AND OF SURFACE-PROTEINS AS AIDS FOR SPECIES IDENTIFICATION OF INSECT TRYPANOSOMATIDS [J].

CAMARGO, EP ;

MATTEI, DM ;

BARBIERI, CL ;

MOREL, CM .

JOURNAL OF PROTOZOOLOGY, 1982, 29 (02) :251-258

[4]

CANESE A, 1978, REV PARAGUAYA MICROB, V13, P3

[5]

CANESE A, 1972, REV PARAGUAYA MICROB, V7, P15

[6] SCHIZODEME ANALYSES OF TRYPANOSOMA-CRUZI ZYMODEMES FROM CHILE [J].

CARRENO, H ;

ROJAS, C ;

AGUILERA, X ;

APT, W ;

MILES, MA ;

SOLARI, A .

EXPERIMENTAL PARASITOLOGY, 1987, 64 (02) :252-260

[7]

CERISOLA JA, 1975, XENODIAGNOSTICO

[8] TRYPANOSOMA-CRUZI FROM THE PARAGUAYAN CHACO - ISOENZYME PROFILES OF STRAINS ISOLATED AT MAKTHLAWAIYA [J].

CHAPMAN, MD ;

BAGGALEY, RC ;

GODFREYFAUSSET, PF ;

MALPAS, TJ ;

WHITE, G ;

CANESE, J ;

MILES, MA .

JOURNAL OF PROTOZOOLOGY, 1984, 31 (03) :482-486

[9]

EBERT F, 1982, TROPENMED PARASITOL, V33, P140

[10]

EBERT F, 1985, TROP MED PARASITOL, V36, P85

← 1 2 3 →