OPTIMIZING MOBILE-PHASE COMPOSITION, ITS FLOW-RATE AND COLUMN TEMPERATURE IN HPLC USING AN EXPERIMENTAL-DESIGN ASSISTED WITH A SIMPLEX-METHOD

被引:16
作者
GUILLAUME, Y
GUINCHARD, C
机构
[1] Laboratoire de Chimie Analytique, UFR des Sciences Médicales et Pharmaceutiques, Besancon, Place St. Jacques
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 16期
关键词
D O I
10.1080/10826079308019701
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this work, a rapid procedure to separate ten compounds in high performance liquid chromatography is presented. The use of an experimental design assisted with a simplex method is proposed to separate these compounds with only thirteen chromatographic analyses to select the mobile phase composition, its flow rate and the column temperature. A flow rate of 0.77 ml/min with a percentage of methanol of 49,95% in the mixture methanol-water and a column temperature of 51.62-degrees-C gave the most efficient separation conditions.
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页码:3457 / 3470
页数:14
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