GROWTH-FACTOR MESSENGER-RNA PROFILES IN UNSTIMULATED HUMAN MONONUCLEAR-CELLS - IDENTIFICATION OF GENES WHICH ARE CONSTITUTIVELY AND VARIABLY EXPRESSED

Quiescent human mononuclear cells (MNC) as studied ex vivo express highly specific mRNA patterns of growth factors: We recently demonstrated that unstimulated MNC constitutively express the genes for the A and B chains of platelet-derived growth factor (PDGF). This expression was down-regulated by dietary ω-3 fatty acids. We now report that unstimulated human MNC express the genes for platelet-dertived endothelial cell growth factor (PD-ECGF), insulin-like growth factor (IGF-1A, -1B) and transforming growth factor-β1 (TGF-β1). In contrast, acidic and basic fibroblast GF (FGFs), insulin-like GF-2 (IGF-2), transforming GF-α (TGF-α) and epidermal GF (EGF) were not expressed in MNC, nor were α- and β-receptors for PDGF. Quantitatively, as measured over a period of six weeks, expression of PD-ECGF was constant, whereas TGF-β1, IGF-1A, and IGF-1B were expressed at varying levels and all independently of each other. Dietary ω-3 fatty acids had no effect on gene expression. Our results also indicate that down-regulation of PDGF gene expression represents a specific and possibly therapeutic effect of dietary fish oil supplementation. © 1993 Academic Press, Inc.