ASPERGILLUS FLAVUS-INDUCED CHITOSANASE IN GERMINATING CORN AND PEANUT SEEDS - ASPERGILLUS-FLAVUS MECHANISM FOR GROWTH DOMINANCE OVER ASSOCIATED FUNGI AND CONCOMITANT AFLATOXIN PRODUCTION

被引：4

作者：

CUERO, RG

OSUJI, GO

机构：

[1] Prairie View A and M University, Texas A and M University System, CARC, Prairie View, TX, 77446-4079

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1995年 / 12卷 / 03期

关键词：

ASPERGILLUS FLAVUS; CHITOSANASE; AFLATOXIN;

D O I：

10.1080/02652039509374333

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Chitosanase, a new class of enzymes with antifungal properties was induced by toxigenic Aspergillus flavus in both germinating corn and peanut seeds. The enzyme was partially purified and fractioned by SDS-Polyacrylamide Gel Electrophoresis activity and copolymerized with chitosan or glycolchitosan as substrate, then quantified by scanning densitometry and a 2-dimensional analysis software program. Chitosanase enzyme was markedly induced by toxigenic A. flavus growing in germinating corn and peanut seeds, as compared to control (water) which showed the lowest activity (almost nil in corn). However, chitosanase induction was higher in seeds treated with chitosan from crustacea. Overall, enzyme activity was higher in peanut than in corn seeds. However, electrophoresed gels from peanut treated with A. flavus or water showed more polypeptides (three and one, respectively) than gels from corn seeds, which only showed one polypeptide for both A. flavus and water treatment. The enzyme molecular weight was estimated to be between 36 000 and 45 000.

引用

页码：479 / 483

页数：5

共 17 条

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[9]

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