ULTRASTRUCTURAL-LOCALIZATION OF EXTRACELLULAR-MATRIX PROTEINS IN LIVER BIOPSIES USING ULTRACRYOMICROTOMY AND IMMONUGOLD LABELING

被引：33

作者：

BURT, AD

GRIFFITHS, MR

SCHUPPAN, D

VOSS, B

MACSWEEN, RNM

机构：

[1] BERGBAU BERUFSGENOSSENSCHAFT,SILIKOSE FORSCHUNGSINST,BOCHUM,GERMANY

[2] FREE UNIV BERLIN,W-1000 BERLIN 33,GERMANY

[3] UNIV GLASGOW,DEPT PATHOL,GLASGOW G12 8QQ,SCOTLAND

来源：

HISTOPATHOLOGY | 1990年 / 16卷 / 01期

关键词：

extracellular matrix proteins; fibrosis; liver;

D O I：

10.1111/j.1365-2559.1990.tb01060.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe a technique for the localization of extracellular matrix proteins in wedge and needle biopsy specimens of human liver. Using ultra‐thin (50–70 nm) sections of puncture perfusion fixed tissue, extracellular matrix proteins were localized using a protein A‐gold labelling procedure. We obtained good preservation of ultrastructural detail and specific labelling for collagen types I, III and IV and fibronectin. The method represents a sensitive means of identifying the extracellular distribution and the cellular origin of these proteins in normal and diseased human liver. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：53 / 58

页数：6

共 22 条

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