SIMPLE METHOD FOR PRODUCING DIFFERENT GROWTH FRACTIONS INVITRO FOR USE IN ANTI-CANCER DRUG STUDIES

被引：6

作者：

BARRANCO, SC

BOLTON, WE

NOVAK, JK

机构：

[1] Division of Cell Biology, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas

来源：

CELL AND TISSUE KINETICS | 1979年 / 12卷 / 01期

关键词：

D O I：

10.1111/j.1365-2184.1979.tb00109.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A simple technique has been used experimentally to produce in vitro Chinese hamster ovary cells with growth fractions ranging from 0 to 100%. Known numbers of exponentially growing and plateau‐phase tissue culture cells were mixed in various proportions to yield the desired final growth fraction. Cells attach to the culture flask surface within 1 hr of mixing. Treatment at that time with the nitrosourea compounds, CCNU and MeCCNU, resulted in differential drug survival sensitivities that were dependent upon the growth fraction of the population treated. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：11 / 16

页数：6

共 14 条

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