A SINGLE AMINO-ACID EXCHANGE ALTERS THE PHARMACOLOGY OF NEONATAL RAT GLYCINE RECEPTOR SUBUNIT

被引：167

作者：

KUHSE, J

SCHMIEDEN, V

BETZ, H

机构：

[1] Zentrum für Molekulare Biologie Heidelberg Universität Heidelberg Im Neuenheimer Feld 282

来源：

NEURON | 1990年 / 5卷 / 06期

关键词：

D O I：

10.1016/0896-6273(90)90346-H

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Agonist activation of the inhibitory glycine receptor (GlyR) in the adult vertebrate CNS is efficiently antagonized by the alkaloid strychnine. Here, we describe a novel rat GlyR alpha subunit cDNA (alpha-2*) that generates chloride channels of low strychnine sensitivity upon expression in Xenopus oocytes. Comparison with the highly homologous human alpha-2 polypeptide and site-directed mutagenesis identified a single amino acid exchange at position 167 that causes the altered pharmacology of alpha2* receptors. Amplification by the polymerase chain reaction revealed a strong decrease in alpha-2* mRNA abundancy during postnatal spinal cord development. These data indicate that alpha-2* represents a ligand binding subunit of the previously identified neonatal GlyR isoform of low strychnine affinity.

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页码：867 / 873

页数：7

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