REDUCED LEVELS OF ADP-RIBOSYLATABLE ELONGATION FACTOR-II IN AGED AND SV40-TRANSFORMED HUMAN CELL-CULTURES

被引：24

作者：

RIIS, B ^{[1
]}

RATTAN, SIS ^{[1
]}

DERVENTZI, A ^{[1
]}

CLARK, BFC ^{[1
]}

机构：

[1] AARHUS UNIV,DEPT CHEM,CELLULAR AGEING LAB,DK-8000 AARHUS,DENMARK

来源：

FEBS LETTERS | 1990年 / 266卷 / 1-2期

关键词：

ADP-ribosylation; Ageing; Diphtheria toxin; Elongation factor-2; Protein synthesis; Transformation;

D O I：

10.1016/0014-5793(90)81502-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The elongation step is involved in the regulation of protein synthesis during the cell cycle, environmental stress, ageing and transformation. Using a diphtheria toxin-mediated assay for measuring the levels of ADP-ribosylatable elongation factor EF-2, we have observed an irreversible decrease of up to 64% in the amount of ADP-ribosylatable EF-2 in normal diploid human fibroblasts MRC-5 undergoing ageing in vitro. However, a similar decrease in low serum-associated G0/G1-arrested cells is reversible both in MRC-5 cells and in their SV40-transformed counterparts. Reduced levels of ADP-ribosylatable EF-2 could account for the slowing-down of protein synthesis during cell cycle arrest and during cellular ageing in culture. © 1990.

引用

页码：45 / 47

页数：3

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