2-BROMOACETYL-6-METHOXYNAPHTHALENE - A USEFUL FLUORESCENT LABELING REAGENT FOR HPLC ANALYSIS OF CARBOXYLIC-ACIDS

被引：23

作者：

GATTI, R ^{[1
]}

CAVRINI, V ^{[1
]}

ROVERI, P ^{[1
]}

机构：

[1] DEPT PHARMACEUT SCI,VIA BELMELORO 6,I-40126 BOLOGNA,ITALY

来源：

CHROMATOGRAPHIA | 1992年 / 33卷 / 1-2期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; FATTY ACIDS AND BILE ACIDS; FLUOROGENIC PRECOLUMN DERIVATIZATION; 2-BROMOACETYL-6-METHOXYNAPHTHALENE;

D O I：

10.1007/BF02276844

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of 2-bromoacetyl-6-methoxynaphthalene as a fluorogenic labelling reagent in pre-column derivatization for the HPLC separation of biologically active carboxylic acids (fatty acids and bile acids) has been investigated. The compound reacts (30 min. at 70-degrees-C) with carboxylic acids to give fluorescent esters that can be separated by reversed-phase HPLC and detected at lambda-ex. 300 nm, lambda-em. 460 nm. The experimental conditions for the derivatization and chromatographic separation are discussed. Applications to the determination of valproic acid and chenodeoxycholic acid in pharmaceutical formulations are described.

引用

页码：13 / 18

页数：6

共 13 条

[1] A REVIEW OF REACTION DETECTION IN HPLC [J].

BRINKMAN, UAT .

CHROMATOGRAPHIA, 1987, 24 :190-200

[2] PHOSPHONIUM TRIBROMIDES - BROMATION AGENTS FROM ORGANIC SUBSTRATES [J].

CRISTAU, HJ ;

TORREILLES, E ;

MORAND, P ;

CHRISTOL, H .

PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS, 1985, 25 (03) :357-367

[3] PRECOLUMN AND POSTCOLUMN DERIVATIZATION CHEMISTRY IN CONJUNCTION WITH HPLC FOR PHARMACEUTICAL ANALYSIS [J].

DANIELSON, ND ;

TARGOVE, MA ;

MILLER, BE .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1988, 26 (08) :362-371

[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALIPHATIC THIOLS WITH AROYLACRYLIC ACIDS AS FLUOROGENIC PRECOLUMN DERIVATIZATION REAGENTS [J].

GATTI, R ;

CAVRINI, V ;

ROVERI, P ;

PINZAUTI, S .

JOURNAL OF CHROMATOGRAPHY, 1990, 507 :451-458

[5] FLUORESCENCE LABELING OF DICARBOXYLIC-ACIDS FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION [J].

GRUSHKA, E ;

LAM, S ;

CHASSIN, J .

ANALYTICAL CHEMISTRY, 1978, 50 (09) :1398-1399

[6] FLUORESCENCE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF FREE AND CONJUGATED BILE-ACIDS IN SERUM AND BILE USING 1-BROMOACETYLPYRENE AS A PRELABELING REAGENT [J].

KAMADA, S ;

MAEDA, M ;

TSUJI, A .

JOURNAL OF CHROMATOGRAPHY, 1983, 272 (01) :29-41

[7]

Lingeman H., 1990, DETECTION ORIENTED D

[8]

MARTINDALE, 1989, EXTRA PHARMACOPOEIA, P400

[9] 1-PYRENYLDIAZOMETHANE AS A FLUORESCENT LABELING REAGENT FOR LIQUID-CHROMATOGRAPHIC DETERMINATION OF CARBOXYLIC-ACIDS [J].

NIMURA, N ;

KINOSHITA, T ;

YOSHIDA, T ;

UETAKE, A ;

NAKAI, C .

ANALYTICAL CHEMISTRY, 1988, 60 (19) :2067-2070

[10] FLUORESCENT LABELING OF FATTY-ACIDS WITH 9-ANTHRYLDIAZOMETHANE (ADAM) FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

NIMURA, N ;

KINOSHITA, T .

ANALYTICAL LETTERS PART A-CHEMICAL ANALYSIS, 1980, 13 (03) :191-202

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