GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC FRACTIONS REFLECTING ARACHIDONIC-ACID METABOLISM IN MOUSE PERITONEAL-MACROPHAGES

被引：9

作者：

ABIAN, J ^{[1
]}

BIOQUE, G ^{[1
]}

BULBENA, O ^{[1
]}

ROSELLO, J ^{[1
]}

GELPI, E ^{[1
]}

机构：

[1] CSIC, CID,DEPT NEUROCHEM,EICOSANOID BRANCH, J GIRONA 18-26, E-08034 BARCELONA, SPAIN

来源：

BIOLOGICAL MASS SPECTROMETRY | 1992年 / 21卷 / 02期

关键词：

D O I：

10.1002/bms.1200210203

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Mouse peritoneal macrophages are used as a model for studies undertaken around the oxidative metabolism of arachidonic acid elicted by xenobiotics (N-phenyllinoleamide, related to the toxic oil syndrome, has been used as an example). A high-performance liquid chromatographic method for cyclo- and lipoxygenase metabolite fractionation has been developed. Gas chromatographic/mass spectrometric analysis of the high-performance liquid chromatographic fractions thus obtained show that the major products detected in the incubates correspond to three principal structures: monohydroxy acids (12-hydroxyeicosatetraenoic acid being the major component), epoxyhydroxy acids and trihydroxy acids. Other minor compounds such as 12-hydroxyheptadecatrienoic acid, various dihydroxy acids and prostaglandins were also detected. Cells pre-exposed to N-phenyllinoleamide show selectively enhanced levels of 6-keto prostaglandin f1-alpha measured by both gas chromatography/mass spectrometry and radioimmunoassay of the corresponding high-performance liquid chromatographic fraction.

引用

页码：69 / 79

页数：11

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