FUNCTIONAL EXPRESSION OF A PLANT PLASMA-MEMBRANE TRANSPORTER IN XENOPUS OOCYTES

被引：36

作者：

BOORER, KJ ^{[1
]}

FORDE, BG ^{[1
]}

LEIGH, RA ^{[1
]}

MILLER, AJ ^{[1
]}

机构：

[1] AFRC,IACR,DEPT BIOCHEM & PHYSIOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND

来源：

FEBS LETTERS | 1992年 / 302卷 / 02期

关键词：

ARABIDOPSIS-THALIANA; HEXOSE TRANSPORTER; PROTON SYMPORTER; HETEROLOGOUS EXPRESSION; XENOPUS OOCYTE;

D O I：

10.1016/0014-5793(92)80431-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length cDNA clone for the H+/hexose co-transporter (STP1) from Arabidopsis thaliana has been transcribed in vitro and the mRNA injected into Xenopus oocytes. Under optimized conditions, oocytes injected with the STP1 mRNA accumulated 3-O-[methyl-C-14]glucose at rates of more than a 1000-fold greater than water-injected control oocytes. A hexose-elicited depolarization of the oocyte membrane potential was demonstrated, and uptake was shown to be stimulated by low external pH, confirming the activity of a H+/hexose co-transport system. This is the first example of the functional expression of a plant membrane transporter in oocytes.

引用

页码：166 / 168

页数：3

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