FETAL CELLS IN THE MATERNAL CIRCULATION - ISOLATION BY MULTIPARAMETER FLOW-CYTOMETRY AND CONFIRMATION BY POLYMERASE CHAIN-REACTION

被引：70

作者：

WACHTEL, S

ELIAS, S

PRICE, J

WACHTEL, G

PHILLIPS, O

SHULMAN, L

MEYERS, C

SIMPSON, JL

DOCKTER, M

机构：

[1] Department of Microbiology and Immunology, University of Tennessee, Memphis

[2] Department of Obstetrics and Gynecology, University of Tennessee, Memphis

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 10期

关键词：

FETAL; ERYTHROCYTES; FLOW; CYTOMETRY; PCR; PRENATAL DIAGNOSIS;

D O I：

10.1093/oxfordjournals.humrep.a137290

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

During pregnancy, nucleated fetal erythrocytes enter the maternal circulation and can be isolated efficiently from the maternal cells by multiparameter flow cytometry. Male DNA, implying presence of a male fetus, can be identified in flow-sorted maternal blood by polymerase chain reaction with oligonucleotide primers flanking single-copy Y-specific DNA sequences. Among flow-sorted samples, we correctly identified fetal sex in 17/18 (94%) pregnancies of 10-21 weeks gestation. Maternal blood thus provides a potential opportunity for prenatal diagnosis that could preclude the need for invasive procedures in current use.

引用

页码：1466 / 1469

页数：4

共 15 条

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