USE OF LOW-MOLECULAR MASS RNA PROFILES TO IDENTIFY LACTIC-ACID BACTERIA AND RELATED ORGANISMS ASSOCIATED WITH FOODS

被引：6

作者：

COLLINSTHOMPSON, DL

SLADE, PJ

GOETHALS, M

机构：

[1] Department of Environmental Biology and Food Science, University of Guelph, Guelph, Ont.

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1991年 / 14卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

IDENTIFICATION METHOD; LACTIC ACID BACTERIA; LOW MOLECULAR MASS RNA PROFILE;

D O I：

10.1016/0168-1605(91)90100-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Fourteen strains of lactic acid bacteria and species of Brochothrix, Carnobacterium, Enterococcus, Erysipelothrix, Kurthia and Listeria were examined using low molecular mass RNA (5S rRNA and tRNA) profiles. These profiles were developed on denaturing polyacrylamide gels. Gel strengths between 9 and 14% were tested to improve resolution of distinct bands for densitometrical analysis. Profiles generated on 12% gels proved to be the best for scanning. Scans of class 2 tRNAs by densitometry showed a characteristic profile for each genus. In the case of Lactobacillus each species studied gave a unique profile. The technique of low molecular mass RNA profiling may provide a useful means for identifying different bacteria from ecosystems such as meats.

引用

页码：135 / 143

页数：9

共 12 条

[1]

FERESU SB, 1988, J GEN MICROBIOL, V134, P1165

[2] IDENTIFICATION OF BACTERIA BY LOW-MOLECULAR WEIGHT RNA PROFILES - A NEW CHEMOTAXONOMIC APPROACH [J].