COUPLING OF ADENOVIRUS TO TRANSFERRIN POLYLYSINE DNA COMPLEXES GREATLY ENHANCES RECEPTOR-MEDIATED GENE DELIVERY AND EXPRESSION OF TRANSFECTED GENES

被引：460

作者：

WAGNER, E ^{[1
]}

ZATLOUKAL, K ^{[1
]}

COTTEN, M ^{[1
]}

KIRLAPPOS, H ^{[1
]}

MECHTLER, K ^{[1
]}

CURIEL, DT ^{[1
]}

BIRNSTIEL, ML ^{[1
]}

机构：

[1] UNIV N CAROLINA, CHAPEL HILL, NC 27599 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 13期

关键词：

GENE THERAPY; ENDOCYTOSIS; ENDOSOME; GENE TRANSFER; DNA TRANSFECTION;

D O I：

10.1073/pnas.89.13.6099

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We are developing efficient methods for gene transfer into tissue culture cells. We have previously shown that coupling of a chimeric adenovirus with polylysine allowed the construction of an adenovirus-polylysine-reporter-gene complex that transferred the transporter gene with great efficiency into HeLa cells. We have now explored simpler, biochemical means for coupling adenovirus to DNA/polylysine complexes and show that such complexes yield virtually 100% transfection in tissue culture cell lines. In these methods adenovirus is coupled to polylysine, either enzymatically through the action of transglutaminase or biochemically by biotinylating adenovirus and streptavidinylating the polylysine moiety. Combination complexes containing DNA, adenovirus-polylysine, and transferrin-polylysine have the capacity to transfer the reporter gene into adenovirus-receptor- and/or transferrin-receptor-rich cells.

引用

页码：6099 / 6103

页数：5

共 17 条

[1] TRANSFERRIN POLYCATION-MEDIATED INTRODUCTION OF DNA INTO HUMAN LEUKEMIC-CELLS - STIMULATION BY AGENTS THAT AFFECT THE SURVIVAL OF TRANSFECTED DNA OR MODULATE TRANSFERRIN RECEPTOR LEVELS [J].