AN INTEGRATED PROCESS FOR THE PRODUCTION AND BIOTRANSFORMATION OF PENICILLIN

被引：22

作者：

BARENSCHEE, T ^{[1
]}

SCHEPER, T ^{[1
]}

SCHUGERL, K ^{[1
]}

机构：

[1] UNIV HANNOVER,INST TECH CHEM,CALLINSTR 3,W-3000 HANNOVER 1,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1992年 / 26卷 / 2-3期

关键词：

PENICILLIN-G; AMPICILLIN; 6-APA; PENICILLIN-G-AMIDASE; ENZYME LIQUID MEMBRANE REACTOR; BIOTRANSFORMATION;

D O I：

10.1016/0168-1656(92)90002-Q

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The extraction of Penicillin G (PG) from the filtered cultivation medium of Penicillium chrysogenum and its conversion into 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PhA) at pH 8 was performed in a 10 1 Kuhni extractor during the production by means of penicillin-G-amidase immobilized in a liquid membrane carrier system (LM). 6-APA was enriched in LM, and the PhA returned to the cultivation medium. After electrocoalescence of LM, the 6-APA was converted into ampicillin with the same enzyme at pH 6, while the liquid membrane phase and enzyme were recycled and reused.

引用

页码：143 / 154

页数：12

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