EICOSANOID BIOSYNTHESIS BY HEMOCYTES FROM THE TOBACCO HORNWORM, MANDUCA-SEXTA

被引：40

作者：

GADELHAK, GG ^{[1
]}

PEDIBHOTLA, VK ^{[1
]}

STANLEYSAMUELSON, DW ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT ENTOMOL,INSECT BIOCHEM & PHYSIOL LAB,LINCOLN,NE 68583

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1995年 / 25卷 / 06期

关键词：

EICOSANOIDS; INSECT IMMUNITY; LIPOXYGENASE; PGH SYNTHASE; MANDUCA SEXTA;

D O I：

10.1016/0965-1748(95)00014-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe eicosanoid biosynthesis by microsomal-enriched preparations of hemocytes from larvae of the tobacco hornworm Manduca sexta. Four major prostaglandins, PGA(2), PGE(2), PGD(2) and PGF(2 alpha), and a lipoxygenase product that co-chromatographed with 15-hydroxyeicosatetraenoic acid (HETE) were synthesized under most conditions. The HETE's fraction was the predominant product. Eicosanoid biosynthesis was sensitive to experimental conditions, including incubation time, temperature, and protein concentration. Optimal biosynthesis was observed with 1.5 mg of microsomal-enriched protein, incubated at 30 degrees C for 2 min. The hemocyte preparation is sensitive to low dosages of naproxin and esculetin. As in mammals, most lipoxygenase activity (87%) was localized in the cytosolic fraction of hemocytes. Unlike mammals, in which PGH synthase is associated with intracellular membranes, the hemocytic activity was detected in microsomal (59%), cytosolic (35%) and mitochondrial fractions (5%).

引用

页码：743 / 749

页数：7

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