MICROWAVE FIXATION AND LOCALIZATION OF CALCIUM IN SYNAPTIC VESICLES

被引：22

作者：

MIZUHIRA, V ^{[1
]}

HASEGAWA, H ^{[1
]}

NOTOYA, M ^{[1
]}

机构：

[1] KANZAKIGAWA RES LAB,SHIONOGI RES LABS,TOYONAKA,OSAKA 561,JAPAN

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1994年 / 55卷 / 02期

关键词：

MICROWAVE FIXATION; OPENING IMAGE; SYNAPTIC VESICLE; CA2+ ION DETECTION; COMPUTERIZED X-RAY MICROANALYSIS;

D O I：

10.1016/0165-0270(94)90205-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The distribution of Ca2+ ions is demonstrated in the synaptic terminals by means of a 2-step chemical precipitation of Ca2+ ions in nervous tissue. K-oxalate/K-antimonate chemical replacement with simultaneous computerized microwave irradiation is used. This precipitate in cell structures was investigated by computerized electron probe X-ray micro-analysis. The calculated values (from the theoretical, standards and sections), elemental binding ratios and elemental molecular weight ratios were compared. Each calculated value coincided with the theoretical value. This method can reliably detect Ca2+ ions at the micromolar level. Ca2+ ions were distributed in the synaptic vesicles and surrounding membranes. Further progress is expected in freeze-substitution and in the application and propagation of the EELS-Imaging system in calcium determinations.

引用

页码：125 / 136

页数：12

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