BROADLY APPLICABLE POLYSILOXANE-BASED CHIRAL STATIONARY-PHASE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SUPERCRITICAL-FLUID CHROMATOGRAPHY

A recently reported broad-spectrum chiral selector and two of its homologs were incorporated into polymethylhydrosiloxane and each of the polymers was immobilized on silica gel to afford chiral stationary phases (CSPs) useful for the separation of the enantiomers of a wide variety of compounds by HPLC and/or supercritical fluid chromatography (SFC). These CSPs are stable toward normal-phase, reversed-phase, and sub/supercritical fluid conditions and are not adversely affected by addition of carboxylic acids or amines as mobile-phase modifiers. These polymeric CSPs show higher efficiencies and shorter analysis times when used with sub/supercritical carbon dioxide then when used in the HPLC mode. Additionally, some separations are achievable using sub/supercritical carbon dioxide but not when the same column is used in the HPLC mode.