STUDIES ON PROTAMINES .13. FRACTIONATION OF CLUPEINE Y

1. More reproducible and satisfactory chromatographic systems on CM-cellulose (see Fig. 2) were found for fractionation of whole clupeine into Y and Z fractions in place of the previous method by adsorption column chromatography on alumina.2. Further fractionation of clupeine Y was tried on a CM-cellulose column using several buffer systems over a wide pH range. Although YI rich and YII rich fractions were obtained, complete separation of these 2 components could not be achieved in the case of intact clupeine Y.3. From the chromatogram of clupeine Y fraction, however, clupeine Y was deduced to consist of nearly equal amounts of 2 main components, YI and YII, i.e. whole clupeine is composed of only 3 main species of molecules, YI, YII and Z. The amino acid compositions other than arginine of clupeine YI and YII were estimated as follows: 2Ala, 3Ser, 2Thr, 2Pro, 1Gly and 1Ile for clupeine YI and 2Ala, 2Ser, 1Thr, 3Pro and 2Val for clupeine YII.4. In the case of whole clupeine modified with trinitrobenzenesulfonic acid, all the 3 components of clupeine were successfully fractionated as TNP-YI, TNP-Z and free YII by one step elution from a CM-cellulose column. © 1968 by the Journal of Biochemistry.