EFFECTIVE PRODUCTION BY ELECTROFUSION OF HYBRIDOMAS SECRETING MONOCLONAL-ANTIBODIES AGAINST HC-ANTIGEN OF SALMONELLA

被引：11

作者：

TSONEVA, I ^{[1
]}

TOMOV, T ^{[1
]}

PANOVA, I ^{[1
]}

STRAHILOV, D ^{[1
]}

机构：

[1] ACAD MED SOFIA, INFECT & PARASIT DIS RES INST, BU-1504 SOFIA, BULGARIA

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1990年 / 24卷 / 01期

关键词：

D O I：

10.1016/0302-4598(80)85005-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hybridomas secreting monoclonal antibodies (Mab) were generated by electrofusion of splenic lymphocytes from Balb/c mice immunized with Hc-antigen of Salmonella with mouse myeloma cells (x63-Ag8.653). The fusion efficiency was expressed by the number of antibody secreting hybridomas/106 lymphocytes. The results obtained show that: (1) the number of antibody secreting hybridomas produced by electrofusion is one order of magnitude higher than with the polyethylene glycol (PEG) technique; (2) pretreatment of lymphocytes with electrical pulses leads to an additional increase in the fusion yield; (3) treatment with protease does not change the yield; (4) electrofusion resulted in both IgG- and IgM-secreting hybridomas. It is supposed that the increased fusion efficiency when using lymphocytes pretreated with electrical pulses is due to their increased “fusogenicity”. © 1990, All rights reserved.

引用

页码：41 / 49

页数：9

共 31 条

[1]

BERG H, 1987, STUD BIOPHYS, V119, P17

[2] HUMAN HYBRIDOMA CELLS PRODUCED BY ELECTRO-FUSION [J].

BISCHOFF, R ;

EISERT, RM ;

SCHEDEL, I ;

VIENKEN, J ;

ZIMMERMAN, U .

FEBS LETTERS, 1982, 147 (01) :64-68

[3] REVERSIBLE PLASMA-MEMBRANE ULTRASTRUCTURAL-CHANGES CORRELATED WITH ELECTROPERMEABILIZATION IN CHINESE-HAMSTER OVARY CELLS [J].

ESCANDEGERAUD, ML ;

ROLS, MP ;

DUPONT, MA ;

GAS, N ;

TEISSIE, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 939 (02) :247-259

[4]

GLASER RW, 1989, STUD BIOPHYS, V130, P201

[5] EFFORTS TO PRODUCE HUMAN CYTOTOXIC T-CELL HYBRIDOMAS BY ELECTROFUSION AND PEG FUSION [J].

GRAVEKAMP, C ;

SANTOLI, D ;

VREUGDENHIL, R ;

COLLARD, JG ;

BOLHUIS, RLH .

HYBRIDOMA, 1987, 6 (02) :121-133

[6] MONOCLONAL ANTI-CYTOKERATIN ANTIBODY FROM A HYBRIDOMA CLONE GENERATED BY ELECTROFUSION [J].

KARSTEN, U ;

PAPSDORF, G ;

ROLOFF, G ;

STOLLEY, P ;

ABEL, H ;

WALTHER, I ;

WEISS, H .

EUROPEAN JOURNAL OF CANCER & CLINICAL ONCOLOGY, 1985, 21 (06) :733-&

[7]

KATENKAMP U, 1989, STUD BIOPHYS, V130, P205

[8] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].

KOHLER, G ;

MILSTEIN, C .

NATURE, 1975, 256 (5517) :495-497

[9] MONOCLONAL-ANTIBODY PRODUCTION BY RECEPTOR-MEDIATED ELECTRICALLY INDUCED CELL-FUSION [J].

LO, MMS ;

TSONG, TY ;

CONRAD, MK ;

STRITTMATTER, SM ;

HESTER, LD ;

SNYDER, SH .

NATURE, 1984, 310 (5980) :792-794

[10] P-31 NMR ANALYSIS OF MEMBRANE PHOSPHOLIPID ORGANIZATION IN VIABLE, REVERSIBLY ELECTROPERMEABILIZED CHINESE-HAMSTER OVARY CELLS [J].

LOPEZ, A ;

ROLS, MP ;

TEISSIE, J .

BIOCHEMISTRY, 1988, 27 (04) :1222-1228

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