IDENTIFICATION OF THE ATRIAL NATRIURETIC FACTOR-R1C RECEPTOR SUBTYPE (B-CLONE) IN CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS

被引:8
作者
FETHIERE, J [1 ]
GRAIHLE, R [1 ]
DELEAN, A [1 ]
机构
[1] UNIV MONTREAL,FAC MED,DEPT PHARMACOL,CP 6128,SUCC A,MONTREAL H3C 3V7,QUEBEC,CANADA
关键词
ATRIAL NATRIURETIC FACTOR; BRAIN NATRIURETIC PEPTIDE; C-TYPE NATRIURETIC PEPTIDE; GUANYLATE CYCLASE; NATRIURETIC FACTOR RECEPTOR;
D O I
10.1016/0014-5793(92)80868-H
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present report demonstrates the presence in cultured rat aortic smooth muscle cells of a natriuretic factor receptor subtype with a specificity typical of the ANF-R1C (B-clone) receptor subtype. To prove the existence of this receptor subtype in this cell line we show that pCNP-(82-103) is the most potent activator of the intrinsic guanylate cyclase activity, and that [I-125]pCNP-(82-103) binds to a specific receptor subtype which is insensitive to the ANF-R2 specific ligand, C-ANF. The investigation of its binding characteristics show the rank potency order of the natriuretic factors in competing for pCNP binding to be pCNP > pBNP > rANF. Furthermore it was possible to covalently photolabel this receptor subtype with underivatized ]I-125]pCNP and show that it is composed of a single subunit of 130 kDa with very high specificity for pCNP.
引用
收藏
页码:77 / 80
页数:4
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