DIFFERENTIAL ALTERATIONS IN MICROVASCULAR PERFUSION IN VARIOUS ORGANS DURING EARLY AND LATE SEPSIS

被引:89
作者
WANG, P
ZHOU, M
RANA, MW
BA, ZF
CHAUDRY, IH
机构
[1] MICHIGAN STATE UNIV,DEPT SURG,SHOCK & TRAUMA RES LABS,B424 CLIN CTR,E LANSING,MI 48824
[2] MICHIGAN STATE UNIV,DEPT PHYSIOL,E LANSING,MI 48824
[3] ST LOUIS UNIV,DEPT ANAT & NEUROBIOL,ST LOUIS,MO 63104
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 01期
关键词
MICROCIRCULATION; CECAL LIGATION AND PUNCTURE; COLLOIDAL CARBON PERFUSION; LASER-DOPPLER FLOWMETRY; HYPERHEMODYNAMIC STATE;
D O I
10.1152/ajpgi.1992.263.1.G38
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Although cellular dysfunction occurs very early in sepsis, it remains controversial whether this is solely due to a decrease in tissue perfusion. Recent studies have indicated that while active hepatocellular function was depressed, hepatic surface microvascular blood flow (MBF) increased in early sepsis but decreased in late sepsis as produced by cecal ligation and puncture (CLP). However, it is not known whether microvascular hyperperfusion in early sepsis and microvascular hypoperfusion in late sepsis are common events in other organs under such conditions. To study this, rats were subjected to sepsis by CLP, after which these and the corresponding shams received 3 ml/100 g body wt normal saline. Microvascular perfusion and MBF in various tissues were assessed by colloidal carbon infusion and laser-Doppler flowmetry, respectively, at 5 h (i.e., early sepsis) or 20 h (late sepsis) after CLP or sham operation. Carbon-perfused areas were quantitated by an Optomax image analyzer. The results indicate that the carbon-perfused areas and MBF in the liver, renal cortex, spleen, and small intestinal serosa (only MBF) increased significantly 5 h after CLP. In late sepsis, however, the carbon-perfused areas and MBF were found to be significantly decreased. A highly linear relationship was observed between the changes of carbon-perfused areas and MBF during sepsis in the tested organs. Thus the microvascular responses in the fluid-resuscitated sepsis model are characterized by hyperperfusion in the early stage and hypoperfusion in the late stage of sepsis in the tested tissues. The cellular dysfunctions observed during the early stage of sepsis are, therefore, not due to any reduction in tissue perfusion.
引用
收藏
页码:G38 / G43
页数:6
相关论文
共 31 条
[1]   ASSESSMENT OF GASTRIC BLOOD-FLOW WITH LASER DOPPLER FLOWMETRY [J].
AHN, H ;
IVARSSON, LE ;
JOHANSSON, K ;
LINDHAGEN, J ;
LUNDGREN, O .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1988, 23 (10) :1203-1210
[2]   ROLE OF GLUCAGON IN SPLANCHNIC HYPEREMIA OF CHRONIC PORTAL-HYPERTENSION [J].
BENOIT, JN ;
ZIMMERMAN, B ;
PREMEN, AJ ;
GO, VLW ;
GRANGER, DN .
AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 251 (05) :G674-G677
[3]   HYPERDYNAMIC SEVERE INTRAVASCULAR SEPSIS DEPENDS ON FLUID ADMINISTRATION IN CYNOMOLGUS MONKEY [J].
CARROLL, GC ;
SNYDER, JV .
AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 243 (01) :R131-R141
[4]  
CHAUDRY IH, 1982, ARCH SURG-CHICAGO, V117, P151
[5]   LASER-DOPPLER MEASUREMENT OF TRACHEAL MUCOSAL BLOOD-FLOW - COMPARISON WITH TRACHEAL ARTERIAL FLOW [J].
CORFIELD, DR ;
DEFFEBACH, ME ;
ERJEFALT, I ;
SALONEN, RO ;
WEBBER, SE ;
WIDDICOMBE, JG .
JOURNAL OF APPLIED PHYSIOLOGY, 1991, 70 (01) :274-281
[6]  
CRYER HM, 1988, CIRC SHOCK, V26, P71
[7]  
DAHN MS, 1990, ARCH SURG-CHICAGO, V125, P441
[8]  
DAHN MS, 1990, SURGERY, V107, P295
[9]   ENDOTOXEMIA IN HUMAN SEPTIC SHOCK [J].
DANNER, RL ;
ELIN, RJ ;
HOSSEINI, JM ;
WESLEY, RA ;
REILLY, JM ;
PARILLO, JE .
CHEST, 1991, 99 (01) :169-175
[10]   THE COMPLEX PATTERN OF CYTOKINES IN SEPSIS - ASSOCIATION BETWEEN PROSTAGLANDINS, CACHECTIN, AND INTERLEUKINS [J].
ERTEL, W ;
MORRISON, MH ;
WANG, P ;
BA, ZF ;
AYALA, A ;
CHAUDRY, IH .
ANNALS OF SURGERY, 1991, 214 (02) :141-148