EXPRESSION OF THE LA-CROSSE M-SEGMENT PROTEINS IN A RECOMBINANT VACCINIA EXPRESSION SYSTEM MEDIATES PH-DEPENDENT CELLULAR FUSION

被引：31

作者：

JACOBY, DR

COOKE, C

PRABAKARAN, I

BOLAND, J

NATHANSON, N

GONZALEZSCARANO, F

机构：

[1] UNIV PENN,MED CTR,DEPT NEUROL,CLIN RES BLDG,422 CURIE BLDG,PHILADELPHIA,PA 19104

[2] UNIV PENN,MED CTR,DEPT MICROBIOL,PHILADELPHIA,PA 19104

[3] UNIV PENN,MED CTR,GRAD GRP MOLEC BIOL,PHILADELPHIA,PA 19104

来源：

VIROLOGY | 1993年 / 193卷 / 02期

关键词：

D O I：

10.1006/viro.1993.1213

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To study the expression of La Crosse virus (LAC) glycaproteins, G1 and G2, we constructed a cDNA copy of the open reading frame (ORF) of the middle RNA segment and expressed it in a recombinant vaccinia virus (VV.ORF). Cells infected with W.ORF expressed G1 and G2 at the cell surface and formed syncytia with a pH profile similar to that of LAC. These experiments provide a system of studying the biological functions of the LAC glycoproteins, including processing, targeting, fusion, receptor binding, and antigenicity. © 1993 Academic Press, Inc.

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页码：993 / 996

页数：4

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共 29 条

[1] GOLGI-COMPLEX LOCALIZATION OF THE PUNTA TORO VIRUS G2-PROTEIN REQUIRES ITS ASSOCIATION WITH THE G1-PROTEIN [J].

CHEN, SY ;

MATSUOKA, Y ;

COMPANS, RW .

VIROLOGY, 1991, 183 (01) :351-365

[2] ASSEMBLY AND POLARIZED RELEASE OF PUNTA TORO VIRUS AND EFFECTS OF BREFELDIN-A [J].

CHEN, SY ;

MATSUOKA, Y ;

COMPANS, RW .

JOURNAL OF VIROLOGY, 1991, 65 (03) :1427-1439

[3] MOLECULAR-BIOLOGY OF THE BUNYAVIRIDAE [J].

ELLIOTT, RM .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :501-522

[4] THE LARGE VIRAL-RNA SEGMENT OF CALIFORNIA SEROGROUP BUNYAVIRUSES ENCODES THE LARGE VIRAL PROTEIN [J].

ENDRES, MJ ;

JACOBY, DR ;

JANSSEN, RS ;

GONZALEZSCARANO, F ;

NATHANSON, N .

JOURNAL OF GENERAL VIROLOGY, 1989, 70 :223-228

[5] THE COMPLETE SEQUENCE OF THE MESSENGER-RNA OF SNOWSHOE HARE BUNYAVIRUS REVEALS THE PRESENCE OF INTERNAL HYDROPHOBIC DOMAINS IN THE VIRAL GLYCOPROTEIN [J].

ESHITA, Y ;

BISHOP, DHL .

VIROLOGY, 1984, 137 (02) :227-240

[6] ORGANIZATION OF THE MIDDLE RNA SEGMENT OF SNOWSHOE HARE BUNYAVIRUS [J].

FAZAKERLEY, JK ;

GONZALEZSCARANO, F ;

STRICKLER, J ;

DIETZSCHOLD, B ;

KARUSH, F ;

NATHANSON, N .

VIROLOGY, 1988, 167 (02) :422-432

[7] VIRUS PARTICLE NUCLEIC-ACIDS AND PROTEINS OF 4 BUNYA-VIRUSES [J].

GENTSCH, J ;

BISHOP, DHL ;

OBIJESKI, JF .

JOURNAL OF GENERAL VIROLOGY, 1977, 34 (FEB) :257-268

[8] SMALL VIRAL-RNA SEGMENT OF BUNYA VIRUSES CODES FOR VIRAL NUCLEOCAPSID PROTEIN [J].

GENTSCH, JR ;

BISHOP, DHL .

JOURNAL OF VIROLOGY, 1978, 28 (01) :417-419

[9] M-VIRAL RNA SEGMENT OF BUNYAVIRUSES CODES FOR 2 GLYCOPROTEINS, G1 AND G2 [J].

GENTSCH, JR ;

BISHOP, DHL .

JOURNAL OF VIROLOGY, 1979, 30 (03) :767-770

[10] LACROSSE BUNYAVIRUS CAN MEDIATE PH-DEPENDENT FUSION FROM WITHOUT [J].

GONZALEZSCARANO, F ;

POBJECKY, N ;

NATHANSON, N .

VIROLOGY, 1984, 132 (01) :222-225