CLONING AND CHARACTERIZATION OF THE GENE ENCODING THE 28-KDA GLUTATHIONE-S-TRANSFERASE OF SCHISTOSOMA-MANSONI

被引：27

作者：

MCNAIR, AT

DISSOUS, C

DUVAUXMIRET, O

CAPRON, A

机构：

[1] Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM 167-CNRS 624, Institut Pasteur, Lille

来源：

GENE | 1993年 / 124卷 / 02期

关键词：

D O I：

10.1016/0378-1119(93)90400-W

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The 28-kDa glutathione S-transferase (GST) of Schistosoma mansoni is considered a possible vaccine candidate for use against this medically important parasite. The gene encoding this molecule has been isolated from a lambdaEMBL4 library by using the corresponding cDNA sequence as a probe. The gene contains four exons and is approximately 5.5 kb in length. Analysis of the 5' flanking region revealed the presence of a consensus AP-1 recognition site, 5'-TGACTCA, between nucleotides -231 and -225. Southern blot analysis suggested the presence of a single gene encoding the 28-kDa GST in the S. mansoni genome.

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页码：245 / 249

页数：5

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