BOP GENE-CLUSTER EXPRESSION IN BACTERIORHODOPSIN-OVERPRODUCING MUTANTS OF HALOBACTERIUM-HALOBIUM

被引:14
作者
SHAND, RF [1 ]
BETLACH, MC [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143
关键词
D O I
10.1128/jb.176.6.1655-1660.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultures were either kept in the dark or exposed to light. All three strains showed similar responses to depleted oxygen tensions and the lack of light: bop gene cluster transcript levels first increased in response to steadily declining oxygen, and once oxygen was depleted, transcript levels decreased and became undetectable within 20 to 40 h. In contrast, each strain responded differently to conditions of depleted oxygen and the presence of light. In the wild-type strain, bop gene cluster transcript levels increased 2.4- to 9.2-fold above the highest levels obtained in the dark. In mutant ET1001, bop gene cluster transcript levels did not increase above the highest levels obtained in the dark. In mutant II-7, bop and brp transcript levels did not increase above the highest levels obtained in the dark, but bat transcript levels increased similar to 5.7-fold. This differing response to identical physiological conditions indicates that the mutations resulting in the bacteriorhodopsin-overproducing
引用
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页码:1655 / 1660
页数:6
相关论文
共 20 条
  • [1] BACTERIO-OPSIN MUTANTS OF HALOBACTERIUM-HALOBIUM
    BETLACH, M
    PFEIFER, F
    FRIEDMAN, J
    BOYER, HW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05): : 1416 - 1420
  • [2] CHARACTERIZATION OF A HALOBACTERIAL GENE AFFECTING BACTERIO-OPSIN GENE-EXPRESSION
    BETLACH, M
    FRIEDMAN, J
    BOYER, HW
    PFEIFER, F
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (20) : 7949 - 7959
  • [3] REGULATION OF THE BACTERIO-OPSIN GENE OF A HALOPHILIC ARCHAEBACTERIUM
    BETLACH, MC
    SHAND, RF
    LEONG, DM
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1989, 35 (01) : 134 - 140
  • [4] BETLACH MC, PROTOCOLS ARCHAEAL
  • [5] GROPP F, IN PRESS P NATL ACAD
  • [6] GROPP F, IN PRESS SYST APPL M
  • [7] ANAEROBIC GROWTH OF HALOBACTERIA
    HARTMANN, R
    SICKINGER, HD
    OESTERHELT, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (07): : 3821 - 3825
  • [8] JAVOR B, 1989, HYPERSALINE ENV MICR, P17
  • [9] THE ROLE OF MESSENGER-RNA DEGRADATION IN THE REGULATED EXPRESSION OF BACTERIAL PHOTOSYNTHESIS GENES
    KLUG, G
    [J]. MOLECULAR MICROBIOLOGY, 1993, 9 (01) : 1 - 7
  • [10] TRANSCRIPTION OF GENES INVOLVED IN BACTERIO-OPSIN GENE-EXPRESSION IN MUTANTS OF A HALOPHILIC ARCHAEBACTERIUM
    LEONG, D
    BOYER, H
    BETLACH, M
    [J]. JOURNAL OF BACTERIOLOGY, 1988, 170 (10) : 4910 - 4915