EXPRESSION OF THE ALPHA-SUBUNIT OF PABA PEPTIDE-HYDROLASE (EC 3.4.24.18) IN MDCK CELLS

被引：35

作者：

GRUNBERG, J ^{[1
]}

DUMERMUTH, E ^{[1
]}

ELDERING, JA ^{[1
]}

STERCHI, EE ^{[1
]}

机构：

[1] UNIV BERN,INST BIOCHEM & MOLEC BIOL,CH-3012 BERN,SWITZERLAND

来源：

FEBS LETTERS | 1993年 / 335卷 / 03期

关键词：

PAPA PEPTIDE HYDROLASE; ASTACIN; MEPRIN; INTESTINE; MDCK; PROCESSING;

D O I：

10.1016/0014-5793(93)80422-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, we report the expression of PPH alpha in the polarized cell line MDCK (Madin Darby canine kidney). In these cells, the enzyme was synthesized in an inactive preform, which upon treatment with trypsin was activated. The enzyme isolated from cell extracts was core-glycosylated and appeared to be retained in the ER as a homodimer. No PPH alpha was detectable on the surface of intact cells by immunofluorescence. However, a complex glycosylated soluble but inactive form was present in the culture medium, suggesting that proteolytic removal of the C-terminal membrane anchoring peptide leads to the secretion of PPH alpha.

引用

页码：376 / 379

页数：4

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