A NEW METHOD FOR BOVINE PEPSINOGEN PURIFICATION - PREPARATION OF A SPECIFIC ANTIBODY

被引：7

作者：

GOMES, MA ^{[1
]}

LIMA, WD ^{[1
]}

PESQUERO, JL ^{[1
]}

机构：

[1] UNIV FED MINAS GERAIS,INST BIOL SCI,DEPT PHYSIOL & BIOPHYS,BIOPHYS LAB,BR-31270901 BELO HORIZONT,MG,BRAZIL

来源：

JOURNAL OF IMMUNOASSAY | 1994年 / 15卷 / 02期

关键词：

BOVINE PEPSINOGEN; PEPSINOGEN ANTISERUM; PEPSINOGEN ELISA;

D O I：

10.1080/15321819408013945

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine pepsinogen was purified from abomasum by ammonium sulphate precipitation and ionic exchange chromatography on DEAE-cellulose and Mono Q columns. Purified pepsinogen was shown to be homogeneous by analytical electrophoresis, having an estimated molecular mass of 46,000 Daltons. The isoeletric point, determined by analytical chromatofocusing was 4.6. Using this pepsinogen preparation to immunize rabbits, a specific antiserum of high titer was obtained.

引用

页码：157 / 170

页数：14

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