A SIMPLE METHOD FOR THE QUANTITATIVE-EVALUATION OF FUNCTIONAL-EFFECTS OF XENOBIOTICS WITH CULTURED-CELLS

被引：5

作者：

LEES, D

PUGNERE, D

CASTEL, A

RAUX, O

TRAVO, P

机构：

[1] Centre de Recherches de Biochimie Macromoléculaire, UPR 9008 CNRS, U 249 INSERM, Université de Montpellier I, Montpellier Cédex, 34033

来源：

CELL BIOLOGY AND TOXICOLOGY | 1994年 / 10卷 / 5-6期

关键词：

CELL CULTURES; SINGLE-CELL FUNCTIONAL QUANTITATIVE STUDIES; NONINVASIVE; NONDESTRUCTIVE IMAGE ANALYSIS;

D O I：

10.1007/BF00755775

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time intervals ranging from 10 s to 6 h or more, over periods of time ranging from a few tens of minutes to 3-4 days. Any aspect of cell physiological behavior can be studied (differentiation-dedifferentiation, migration, division, degeneration, death) without preliminary staining and/or fixation provided it results in optically visible changes.

引用

页码：305 / 309

页数：5