STRUCTURAL STUDIES OF O-SPECIFIC POLYSACCHARIDE CHAINS OF THE LIPOPOLYSACCHARIDE FROM YERSINIA-ENTEROCOLITICA SEROVAR 0/10

被引：13

作者：

GORSHKOVA, RP ^{[1
]}

ISAKOV, VV ^{[1
]}

KALMYKOVA, EN ^{[1
]}

OVODOV, YS ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,PACIFIC INST BIOORGAN CHEM,VLADIVOSTOK 690022,RUSSIA

来源：

CARBOHYDRATE RESEARCH | 1995年 / 268卷 / 02期

关键词：

YERSINIA ENTEROCOLITICA; LIPOPOLYSACCHARIDE; O-SPECIFIC POLYSACCHARIDE; D-RHAMNAN; L-XYLULOSE (L-THREO-PENT-2-ULOSE);

D O I：

10.1016/0008-6215(94)00339-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipopolysaccharide (LPS) was isolated from Yersinia enterocolitica serovars O:10 and O:10 KL and the structural pattern of O-specific sugar chains elucidated. The rhamnan and L-xylulose (L-threo-pent-2-ulose) as constituents of the O-specific polysaccharide were obtained by autohydrolysis of the LPS. The rhamnan was shown to be a linear, alpha-(1 --> 3)-linked polysaccharide in the D configuration. L-Xylulose was purified using paper chromatography on a preparative scale and its structure was confirmed by C-13 NMR spectroscopy. Using sugar and methylation analysis and C-13 NMR spectroscopy of the LPS and the rhamnan, the structural features of the disaccharide repeating unit of the Y. enterocolitica O:10 O-specific polysaccharide were elucidated as: GRAPHICS

引用

页码：249 / 255

页数：7

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