SPECTROPHOTOMETRIC DETERMINATION OF ETHANOL IN BLOOD USING A FLOW-INJECTION SYSTEM WITH AN IMMOBILIZED ENZYME (ALCOHOL-DEHYDROGENASE) REACTOR COUPLED TO AN ONLINE DIALYZER

被引：13

作者：

MAEDER, G ^{[1
]}

VEUTHEY, JL ^{[1
]}

PELLETIER, M ^{[1
]}

HAERDI, W ^{[1
]}

机构：

[1] UNIV GENEVA,DEPT INORGAN ANALYT & APPL CHEM,30 QUAI ERNEST ANSERMET,CH-1211 GENEVA 4,SWITZERLAND

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 231卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)86404-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The determination of ethanol in whole blood without pretreatment using spectrotometric detection with an immobilized enzyme reactor coupled to an on-line dialyser is described. Optimum working conditions are given. The effects of the injection volume and the flow-rate on the peak height using the dialyser were investigated. A good linear calibration graph over the ethanol concentration range 3-40 μg ml-1 was observed; these concentrations correspond to 0.3-4.0 g of ethanol per 1000 g of whole blood prior to dilution. For comparison, whole blood samples were analysed by gas chromatography with direct injection. The effect of temperature on the peak height was also studied in a system without the dialyser. © 1990.

引用

页码：115 / 119

页数：5

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