ANTIBODY-PRODUCTION IN PACKED-BED REACTORS USING SERUM-FREE AND PROTEIN-FREE MEDIUM

被引：12

作者：

BLIEM, R

OAKLEY, R

MATSUOKA, K

VARECKA, R

TAIARIOL, V

机构：

[1] Baxter Hyland Division, Hayward, 94545, CA

来源：

CYTOTECHNOLOGY | 1990年 / 4卷 / 03期

关键词：

ANIMAL CELL CULTURE; HYBRIDOMA; MONOCLONAL ANTIBODY; PACKED BED REACTOR; CONTINUOUS CULTURE; PERFUSION; PROTEIN-FREE MEDIUM;

D O I：

10.1007/BF00563788

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present work demonstrates the utility of packed bed reactors for the production of monoclonal antibody. We present data from a continuous process run for the production of over 100 grams of antibody, using serum-free medium. An additional pilot run also demonstrates the potential for continued antibody production under protein-free conditions, using a standard basal medium.

引用

页码：279 / 283

页数：5

共 4 条

[1]

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[2]

Spier R.E., Whiteside J.P., The production of Foot- and Mouth disease virus from BHK 21 C13 cells grown on the surface of glass spheres, 18, pp. 649-657, (1976)

[3]

Griffiths J.B., Cameron D.R., Looby D., A comparison of unit process systems for anchorage-dependent cells, Dev. Biol. Standard., 66, pp. 331-338, (1987)

[4]

Murdin A.D., Thorpe J.S., Groves D.J., Spier R.E., Growth and metabolism of hybriomas immobilized in packed beds: Comparison with static and suspension cultures, Enzyme Microb. Technol., 11, pp. 341-346, (1989)

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