ABSOLUTE QUANTITIES AND EQUILIBRIUM KINETICS OF MACROMOLECULAR ADSORPTION MEASURED BY FLUORESCENCE PHOTOBLEACHING IN TOTAL INTERNAL-REFLECTION

被引：26

作者：

ZIMMERMANN, RM ^{[1
]}

SCHMIDT, CF ^{[1
]}

GAUB, HE ^{[1
]}

机构：

[1] TECH UNIV MUNICH,DEPT PHYS,W-8046 GARCHING,GERMANY

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 1990年 / 139卷 / 01期

关键词：

D O I：

10.1016/0021-9797(90)90462-W

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A combination of total internal reflection fluorescence and fluorescence recovery after photobleaching was used to measure the adsorption of 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) labeled bovine serum albumin and NBD labeled lysozyme to plain quartz and alkylated quartz. A new method was developed to measure quantitatively the adsorbed amount of protein, using an intrinsic calibration. The results were confirmed by accompanying ellipsometry measurements. Equilibrium exchange kinetics of the adsorbed proteins between the surface and the bulk were measured as a function of temperature. © 1990.

引用

页码：268 / 280

页数：13

共 28 条

[1]

ARNEBRANT T, 1985, PROG COLL POL SCI S, V70, P62

[2] TOTAL INTERNAL-REFLECTION FLUORESCENCE [J].