A NEW FAMILY OF SMALL (5 KDA) PROTEIN INHIBITORS OF INSECT ALPHA-AMYLASES FROM SEEDS OR SORGHUM (SORGHUM-BICOLOR (L) MOENCH) HAVE SEQUENCE HOMOLOGIES WITH WHEAT GAMMA-PUROTHIONINS

被引：198

作者：

BLOCH, C ^{[1
]}

RICHARDSON, M ^{[1
]}

机构：

[1] UNIV DURHAM,DEPT BIOL SCI,SCI LABS,S RD,DURHAM DH1 3LE,ENGLAND

来源：

FEBS LETTERS | 1991年 / 279卷 / 01期

关键词：

SORGHUM ALPHA-AMYLASE INHIBITOR; AMINO ACID SEQUENCE; HOMOLOGY WITH GAMMA-PUROTHIONIN;

D O I：

10.1016/0014-5793(91)80261-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three isoinhibitors of locust and cockroach gut alpha-amylases were purified from seeds of sorghum by saline extraction, precipitation with ammonium sulphate, affinity chromatography on Red-Sepharose and preparative RP-HPLC on Vydac C18. The complete primary structures were determined by automatic degradation of the intact reduced and S-alkylated proteins, and by manual DABITC/PITC microsequencing of peptides obtained from enzymes digests. The inhibitors consist of 47 (SI-alpha-1) or 48 (SI-alpha-2, ST-alpha-3) amino acids, and are the smallest plant inhibitors of alpha-amylase currently known. The sequences of the three isoinhibitors exhibit between 38% and 87% identify amoung themselves and also have homology (32-81%) with the gamma-purothionins recently isolated from wheat endosperm.

引用

页码：101 / 104

页数：4

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