INDIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION OF EEL SERUM ANTIBODY

被引：8

作者：

IIDA, T

YONEKURA, H

IZUMIYAMA, M

WAKABAYASHI, H

机构：

[1] Department of Fisheries, Faculty of Agriculture, University of Tokyo, Tokyo 113, Yayoi 1-1-1, Bunkyo-ku

来源：

FISH PATHOLOGY | 1991年 / 26卷 / 04期

关键词：

D O I：

10.3147/jsfp.26.201

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Immunoglobulin (Ig) was purified from the serum of eel (Anguilla japonica) and an indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of eel antibody. Sera were collected from eel which had been injected with formalin-killed Edwardsiella tarda bacterin. A pool of the sera was precipitated with a 40% saturated ammonium sulfate solution, and then immunoglobulin was purified from the precipitates by gel-filtration and ion-exchange chromatography. The purified Ig emulsified in Freund's complete adjuvant was administered to a rabbit to prepare anti-eel Ig serum. Eel were immersed in the E. tarda bacterin at a density of 1 mg wet weight/ml for 1 h, Change in antibody titers in the vaccinated fish was determined by indirect ELISA. The liters of vaccinated fish were significantly higher than those of unvaccinated control fish.

引用

页码：201 / 205

页数：5

共 7 条

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