BRANCH, MICROPROPAGULE AND TISSUE-CULTURE OF THE RED ALGAE EUCHEUMA-DENTICULATUM AND KAPPAPHYCUS-ALVAREZII FARMED IN THE PHILIPPINES

被引:68
作者
DAWES, CJ [1 ]
KOCH, EW [1 ]
机构
[1] UNIV S FLORIDA,DEPT MARINE SCI,ST PETERSBURG,FL 33701
关键词
EUCHEUMA; KAPPAPHYCUS; CULTURE; MICROPROPAGATION; CALLUS REGENERATION;
D O I
10.1007/BF00003583
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Three forms of the iota-producing carrageenophyte, Eucheuma denticulatum, and four forms of the kappa-producing carrageenophyte, Kappaphycus alvarezii, obtained from seaweed farms in the Philippines have been grown in the laboratory under unialgal and axenic conditions. Comparison of media indicates that seed stocks of both species can be cultured using enriched seawater media ranging from ESS and SWMD-1 to inexpensive soil extract (Erdshreiber's) or holding in sterile seawater for up to three weeks. Micropropagation has been successful with at least two forms of each species resulting in clonal propagation from axenic explants within 4 to 8 weeks. Callus development and branch regeneration has also been induced in two forms of each species. The results indicate that culture facilities in the farming areas of the Philippines could maintain high-yielding and rapidly growing seed stock for the seaweed farmers.
引用
收藏
页码:247 / 257
页数:11
相关论文
共 22 条
[1]  
AZANZA-CORRALES R, 1990, Journal of Applied Phycology, V2, P57, DOI 10.1007/BF02179769
[2]   WOUND-HEALING IN CULTURED EUCHEUMA-ALVAREZII VAR TAMBALANG DOTY [J].
AZANZACORRALES, R ;
DAWES, CJ .
BOTANICA MARINA, 1989, 32 (03) :229-234
[3]   SOME EFFECTS OF PLANT-GROWTH REGULATORS ON TISSUE-CULTURES OF THE MARINE RED ALGA AGARDHIELLA-SUBULATA (GIGARTINALES, RHODOPHYTA) [J].
BRADLEY, PM ;
CHENEY, DP .
HYDROBIOLOGIA, 1990, 204 :353-360
[4]   MEDULLARY TISSUE-CULTURE OF RED ALGA CHONDRUS-CRISPUS [J].
CHEN, LCM ;
TAYLOR, ARA .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1978, 56 (07) :883-886
[5]  
CHENEY D, 1986, NOVA HEDWIGIA S, V83, P22
[6]  
CHENEY DP, 1987, P INT SEAWEED S, V12, P161
[7]  
DAWES CJ, 1979, P INT SEAWEED S, V9, P199
[8]  
Doty MS., 1985, TAXONOMY EC SEAWEEDS, V1, P37
[9]  
DOTY MS, 1985, TAXONOMY EC SEAWEEDS, P63
[10]  
GARCIAREINA G, 1988, ALGAL BIOTECHNOLOGY, P111