A CDC2-RELATED KINASE PSSALRE/CDK5 IS HOMOLOGOUS WITH THE 30 KDA SUBUNIT OF TAU-PROTEIN KINASE-II, A PROLINE-DIRECTED PROTEIN-KINASE ASSOCIATED WITH MICROTUBULE

被引：209

作者：

KOBAYASHI, S ^{[1
]}

ISHIGURO, K ^{[1
]}

OMORI, A ^{[1
]}

TAKAMATSU, M ^{[1
]}

ARIOKA, M ^{[1
]}

IMAHORI, K ^{[1
]}

UCHIDA, T ^{[1
]}

机构：

[1] MITSUBISHI KASEI INST LIFE SCI,MACHIDA,TOKYO 194,JAPAN

来源：

FEBS LETTERS | 1993年 / 335卷 / 02期

关键词：

TAU PROTEIN; PAIRED HELICAL FILAMENT; ALZHEIMERS DISEASE; CDC2-RELATED PROTEIN KINASE; CYCLIN-DEPENDENT KINASE 5;

D O I：

10.1016/0014-5793(93)80723-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously reported that tau protein kinase II (TPKII) from bovine brain was composed of 30 kDa and 23 kDa subunits. The 30 kDa subunit of TPKII can be regarded as a catalytic subunit because of its ATP-binding activity. Antibodies directed against TPKII-phosphorylated tau also reacted with tau phosphorylated by cdc2 kinase obtained from starfish oocytes, indicating that TPKII and cdc2 kinase phosphorylate the same sites. We determined the amino acid sequence of the 30 kDa subunit and found it to be homologous with a cdc2-related kinase, PSSALRE/cdk5. Moreover, an antibody against PSSALRE/cdk5 reacted with the 30 kDa subunit. These results indicate that the 30 kDa subunit of TPKII is bovine homologue of PSSALRE/cdk5. Expression of the 30 kDa subunit mRNA was enhanced in juvenile rat brain. This result supports our previous hypothesis that the kinase works actively in juvenile brain.

引用

页码：171 / 175

页数：5

共 37 条

[1] TAU-PROTEIN KINASE-II IS INVOLVED IN THE REGULATION OF THE NORMAL PHOSPHORYLATION STATE OF TAU-PROTEIN
ARIOKA, M
TSUKAMOTO, M
ISHIGURO, K
KATO, R
SATO, K
IMAHORI, K
UCHIDA, T
[J]. JOURNAL OF NEUROCHEMISTRY, 1993, 60 (02) : 461 - 468
[2] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[3] MITOGEN ACTIVATED PROTEIN (MAP) KINASE TRANSFORMS TAU-PROTEIN INTO AN ALZHEIMER-LIKE STATE
DREWES, G
LICHTENBERGKRAAG, B
DORING, F
MANDELKOW, EM
BIERNAT, J
GORIS, J
DOREE, M
MANDELKOW, E
[J]. EMBO JOURNAL, 1992, 11 (06) : 2131 - 2138
[4] CLONING AND SEQUENCING OF THE CDNA-ENCODING A CORE PROTEIN OF THE PAIRED HELICAL FILAMENT OF ALZHEIMER-DISEASE - IDENTIFICATION AS THE MICROTUBULE-ASSOCIATED PROTEIN TAU
GOEDERT, M
WISCHIK, CM
CROWTHER, RA
WALKER, JE
KLUG, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) : 4051 - 4055
[5] MULTIPLE ISOFORMS OF HUMAN MICROTUBULE-ASSOCIATED PROTEIN-TAU - SEQUENCES AND LOCALIZATION IN NEUROFIBRILLARY TANGLES OF ALZHEIMERS-DISEASE
GOEDERT, M
SPILLANTINI, MG
JAKES, R
RUTHERFORD, D
CROWTHER, RA
[J]. NEURON, 1989, 3 (04) : 519 - 526
[6] P42 MAP KINASE PHOSPHORYLATION SITES IN MICROTUBULE-ASSOCIATED PROTEIN TAU ARE DEPHOSPHORYLATED BY PROTEIN PHOSPHATASE-2A1 - IMPLICATIONS FOR ALZHEIMERS-DISEASE
GOEDERT, M
COHEN, ES
JAKES, R
COHEN, P
[J]. FEBS LETTERS, 1992, 312 (01): : 95 - 99
[7] THE ABNORMAL PHOSPHORYLATION OF TAU-PROTEIN AT SER-202 IN ALZHEIMER-DISEASE RECAPITULATES PHOSPHORYLATION DURING DEVELOPMENT
GOEDERT, M
JAKES, R
CROWTHER, RA
SIX, J
LUBKE, U
VANDERMEEREN, M
CRAS, P
TROJANOWSKI, JQ
LEE, VMY
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (11) : 5066 - 5070
[8] THE ALZHEIMER-LIKE PHOSPHORYLATION OF TAU-PROTEIN REDUCES MICROTUBULE BINDING AND INVOLVES SER-PRO AND THR-PRO MOTIFS
GUSTKE, N
STEINER, B
MANDELKOW, EM
BIERNAT, J
MEYER, HE
GOEDERT, M
MANDELKOW, E
[J]. FEBS LETTERS, 1992, 307 (02): : 199 - 205
[9] GLYCOGEN-SYNTHASE KINASE-3 INDUCES ALZHEIMERS DISEASE-LIKE PHOSPHORYLATION OF TAU - GENERATION OF PAIRED HELICAL FILAMENT EPITOPES AND NEURONAL LOCALIZATION OF THE KINASE
HANGER, DP
HUGHES, K
WOODGETT, JR
BRION, JP
ANDERTON, BH
[J]. NEUROSCIENCE LETTERS, 1992, 147 (01) : 58 - 62
[10] HASEGAWA M, 1992, J BIOL CHEM, V267, P17047

← 1 2 3 4 →