THE 3 BACILLUS-ANTHRACIS TOXIN GENES ARE COORDINATELY REGULATED BY BICARBONATE AND TEMPERATURE

被引：106

作者：

SIRARD, JC ^{[1
]}

MOCK, M ^{[1
]}

FOUET, A ^{[1
]}

机构：

[1] INST PASTEUR, GENET MOLEC TOXINES LAB, CNRS, URA 1858, F-75724 PARIS 15, FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 16期

关键词：

D O I：

10.1128/jb.176.16.5188-5192.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The two Bacillus anthracis toxins are composed of three proteins, protective antigen, lethal factor, and edema factor. The structural genes for these three components are located on the virulence plasmid pXO1. We constructed transcriptional fusions between the regulatory region of each of these genes and lacZ. Each construct was then inserted as a single copy at the corresponding toxin gene locus on pXO1, resulting in three isogenic strains. Two environmental factors, bicarbonate and temperature, were found to induce beta-galactosidase synthesis in each recombinant strain. Furthermore, the transcription of the three toxin genes appears to be coordinately regulated.

引用

页码：5188 / 5192

页数：5

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