THE DYNAMICS OF CHROMATIN CONDENSATION - REDISTRIBUTION OF TOPOISOMERASE-II IN THE 87A7 HEAT-SHOCK LOCUS DURING INDUCTION AND RECOVERY

被引：30

作者：

UDVARDY, A ^{[1
]}

SCHEDL, P ^{[1
]}

机构：

[1] PRINCETON UNIV, DEPT MOLEC BIOL, PRINCETON, NJ 08544 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1993年 / 13卷 / 12期

关键词：

D O I：

10.1128/MCB.13.12.7522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the in vivo sites of action for topoisomerases II in the 87A7 heat shock locus as a function of gene activity. When the hsp70 genes are induced, there is a dramatic redistribution of topoisomerase II in the locus which parallels many of the observed alterations in chromatin structure. In addition to changes in the topoisomerase II distribution within the locus, we find topoisomerase II localized around the putative domain boundaries scs and scs'. During recovery, when the chromatin fiber of the locus recondenses, the major sites of action for topoisomerase II appear to be located within the two hsp70 genes and in the intergenic spacer separating the two genes.

引用

页码：7522 / 7530

页数：9

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