SEQUENCE-ANALYSIS OF THE POTENT MITOGENIC TOXIN OF PASTEURELLA-MULTOCIDA

被引：38

作者：

LAX, AJ ^{[1
]}

CHANTER, N ^{[1
]}

PULLINGER, GD ^{[1
]}

HIGGINS, T ^{[1
]}

STADDON, JM ^{[1
]}

ROZENGURT, E ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,LONDON WC2A 3PX,ENGLAND

来源：

FEBS LETTERS | 1990年 / 277卷 / 1-2期

关键词：

MITOGEN; TOXIN; PROTEIN KINASE-C; PASTEURELLA-MULTOCIDA;

D O I：

10.1016/0014-5793(90)80809-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no evidence for a signal sequence. The gene had a low G + C base ratio which differs from the rest of the Pasteurella genome. There was no significant homology with other known proteins, although a motif found in certain bacterial toxins which are ADP-ribosyl transferases is present. A recombinant expressing only part of the PMT gene was not mitogenic.

引用

页码：59 / 64

页数：6

共 26 条

[1]

ANDRESEN LO, 1990, 11TH P IPVS C LAUS, P60

[2] SEQUENCE OF THE DERMONECROTIC TOXIN OF PASTEURELLA-MULTOCIDA SSP MULTOCIDA [J].