SCANNING-TRANSMISSION ELECTRON-MICROSCOPIC MASS DETERMINATION OF INVITRO SELF-ASSEMBLED SMOOTH-MUSCLE MYOSIN-FILAMENTS

被引：9

作者：

CROSS, RA ^{[1
]}

ENGEL, A ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,ME MULLER INST HIGH RESOLUT ELECTRON MICROSCOPY,CH-4056 BASEL,SWITZERLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 03期

关键词：

MYOSIN ASSEMBLY; COILED-COIL ASSEMBLY; MYOSIN FILAMENT; SIDE-POLAR; STEM;

D O I：

10.1016/0022-2836(91)90487-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have measured the mass per unit length of in vitro self-assembled smooth muscle myosin filaments, using scanning transmission electron microscope darkfield images of freeze-dried samples. The measured values were integral multiples, usually 1, 2, 3 or 4, of 75 kDa per nm. The data corroborate an earlier proposal, that these filaments are built from monolayer sheets of molecules, each sheet having two antiparallel myosin molecules per 14.3 nm of its length. © 1991.

引用

页码：455 / 458

页数：4

共 8 条

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