LIPIDS OF FROG RETINAL-PIGMENT EPITHELIUM - COMPARISON WITH ROD OUTER SEGMENTS, RETINA, PLASMA AND RED-BLOOD-CELLS

被引:14
作者
CHEN, HM
ANDERSON, RE
机构
[1] BAYLOR COLL MED, CULLEN EYE INST, 1 BAYLOR PLAZA, HOUSTON, TX 77030 USA
[2] BAYLOR COLL MED, DEPT BIOCHEM, HOUSTON, TX 77030 USA
关键词
D O I
10.3109/02713689209000752
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The glycerolipid and fatty acid compositions of frog retinal pigment epithelium (RPE) were determined and compared with rod outer segments (ROS), retina, plasma, and red blood cells (RBC). The glycerolipid class composition of RPE was similar to RBC and ROS or retina, with phosphatidylcholine and phosphatidylethanolamine being the major components. The fatty acid composition of RPE differed substantially from that of plasma or RBC, the former contained much higher levels of C-20 and C-22 polyunsaturated fatty acids (PUFAs), such as 20:4n-6 and 22:6n-3, but less C-18 mono-, dienoic, and trienoic acids. The difference between RPE and ROS or retina with respect to fatty acid profile was also dramatic; RPE had relatively less 22:6n-3, but more 20:4n-6 and 18:2n-6, than ROS or retina. These results suggest that frog RPE cells may selectively take up C-20 and C-22 PUFAs from the circulation, but preferentially deliver 22:6n-3 to the ROS and retina. Fatty acid analyses show that 20:4n-6 and 22:6n-3 were unevenly distributed among RPE glycerolipids; phosphatidic acid, diglyceride, triglyceride, and phosphatidylserine are relatively more enriched in 22:6n-3 compared with 20:4n-6. This information might imply that these two PUFAs are metabolized differently inside the frog RPE cells.
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页码:793 / 800
页数:8
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