ECTOPIC EXPRESSION OF PMADS3 IN TRANSGENIC PETUNIA PHENOCOPIES THE PETUNIA BLIND MUTANT

被引:127
作者
TSUCHIMOTO, S [1 ]
VANDERKROL, AR [1 ]
CHUA, NH [1 ]
机构
[1] ROCKEFELLER UNIV,PLANT MOLEC BIOL LAB,1230 YORK AVE,NEW YORK,NY 10021
关键词
D O I
10.1105/tpc.5.8.843
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We cloned a MADS-box gene, pMADS3, from Petunia hybrida, which shows high sequence homology to the Arabidopsis AGAMOUS and Antirrhinum PLENA. pMADS3 is expressed exclusively in stamens and carpels of wild-type petunia plants. In the petunia mutant blind, which shows homeotic conversions of corolla limbs into antheroid structures with pollen grains and small parts of sepals into carpelloid tissue, pMADS3 is expressed in all floral organs as well as in leaves. Ectopic expression of pMADS3 in transgenic petunia leads to phenocopies of the blind mutant, i.e., the formation of antheroid structures on limbs and carpelloid tissue on sepals. Transgenic tobacco plants that overexpress pMADS3 exhibit an even more severe phenotype, with the sepals forming a carpel-like structure encasing the interior floral organs. Our results identify BLIND as a negative regulator of pMADS3, which specifies stamens and carpels during petunia flower development.
引用
收藏
页码:843 / 853
页数:11
相关论文
共 24 条
  • [1] DIFFERENTIAL EXPRESSION OF 2 MADS BOX GENES IN WILD-TYPE AND MUTANT PETUNIA FLOWERS
    ANGENENT, GC
    BUSSCHER, M
    FRANKEN, J
    MOL, JNM
    VANTUNEN, AJ
    [J]. PLANT CELL, 1992, 4 (08) : 983 - 993
  • [2] A NUCLEAR GENE ENCODING THE BETA-SUBUNIT OF THE MITOCHONDRIAL ATP-SYNTHASE IN NICOTIANA-PLUMBAGINIFOLIA
    BOUTRY, M
    CHUA, NH
    [J]. EMBO JOURNAL, 1985, 4 (09) : 2159 - 2165
  • [3] BOWMAN JL, 1991, DEVELOPMENT, V112, P1
  • [4] GENES DIRECTING FLOWER DEVELOPMENT IN ARABIDOPSIS
    BOWMAN, JL
    SMYTH, DR
    MEYEROWITZ, EM
    [J]. PLANT CELL, 1989, 1 (01) : 37 - 52
  • [5] COMPLEMENTARY FLORAL HOMEOTIC PHENOTYPES RESULT FROM OPPOSITE ORIENTATIONS OF A TRANSPOSON AT THE PLENA-LOCUS OF ANTIRRHINUM
    BRADLEY, D
    CARPENTER, R
    SOMMER, H
    HARTLEY, N
    COEN, E
    [J]. CELL, 1993, 72 (01) : 85 - 95
  • [6] FLORAL HOMEOTIC MUTATIONS PRODUCED BY TRANSPOSON-MUTAGENESIS IN ANTIRRHINUM-MAJUS
    CARPENTER, R
    COEN, ES
    [J]. GENES & DEVELOPMENT, 1990, 4 (09) : 1483 - 1493
  • [7] DEVLAMING P, 1984, PLANT MOL BIOL REP, V2, P21
  • [8] NEGATIVE REGULATION OF THE ARABIDOPSIS HOMEOTIC GENE AGAMOUS BY THE APETALA2 PRODUCT
    DREWS, GN
    BOWMAN, JL
    MEYEROWITZ, EM
    [J]. CELL, 1991, 65 (06) : 991 - 1002
  • [9] CHARACTERIZATION OF 2 NEW GENES ESSENTIAL FOR VEGETATIVE GROWTH IN SACCHAROMYCES-CEREVISIAE - NUCLEOTIDE-SEQUENCE DETERMINATION AND CHROMOSOME MAPPING
    DUBOIS, E
    BERCY, J
    DESCAMPS, F
    MESSENGUY, F
    [J]. GENE, 1987, 55 (2-3) : 265 - 275
  • [10] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS
    HORSCH, RB
    FRY, JE
    HOFFMANN, NL
    EICHHOLTZ, D
    ROGERS, SG
    FRALEY, RT
    [J]. SCIENCE, 1985, 227 (4691) : 1229 - 1231