NEUROTROPHIN INDUCED CAMP AND IP(3) RESPONSES IN PC12 CELLS - DIFFERENT PATHWAYS

被引：49

作者：

KNIPPER, M ^{[1
]}

BECK, A ^{[1
]}

RYLETT, J ^{[1
]}

BREER, H ^{[1
]}

机构：

[1] UNIV HOHENHEIM, INST ZOOPHYSIOL, W-7000 STUTTGART 70, GERMANY

来源：

FEBS LETTERS | 1993年 / 324卷 / 02期

关键词：

NGF; BDNF; CAMP; IP(3); P75(NGFR); TRK(NGFR);

D O I：

10.1016/0014-5793(93)81382-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NGF and BDNF elevate cAMP and IP3 levels in membranes of PC12 cells within a subsecond time period. The cAMP formation induced by NGF and BDNF pretreatment for 2 s was reduced by GDP-beta-S and PTX, but not the trk(NGFR) inhibitor K 252a. NGF, but not BDNF, induced IP3 formation. IP3 formation was reduced by K 252a, but not by GDP-beta-S and PTX. Using p75NGFR expressing, but trk(NGFR)-deficient PCNA cell membranes, NGF and BDNF induced cAMP formation, but not IP, formation. We suggest that NGF and BDNF induced cAMP formation is mediated via a p75 NGFR/G-protein mediated mechanism, and IP3 formation via a K 252a sensitive pathway.

引用

页码：147 / 152

页数：6

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