THE 5' UNTRANSLATED REGION OF THE HUMAN GAMMA-GLUTAMYL-TRANSFERASE MESSENGER-RNA CONTAINS A TISSUE-SPECIFIC ACTIVE TRANSLATIONAL ENHANCER

被引：21

作者：

DIEDERICH, M

WELLMAN, M

VISVIKIS, A

PUGA, A

SIEST, G

机构：

[1] CTR MED, CNRS, URA 597, 30 RUE LIONNOIS, F-54000 NANCY, FRANCE

[2] UNIV CINCINNATI, DEPT ENVIRONM HLTH, CINCINNATI, OH 45267 USA

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

GAMMA-GLUTAMYL-TRANSFERASE; 5' UNTRANSLATED REGION; TRANSIENT EXPRESSION; RNASE PROTECTION ANALYSIS; TRANSLATIONAL REGULATION; SECONDARY STRUCTURE;

D O I：

10.1016/0014-5793(93)80490-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the functional and structural analysis of the 5' untranslated region (5'UTR) of human hepatoma HepG2 gamma-glutamyltransferase (GGT) mRNA. Transient expression of a hybrid GGT-luciferase gene in HepG2, MIA-Pa-Ca-2 and MG 63 cell lines shows that this 5'UTR acts as a tissue-specific translational enhancer. Evidence for transcripts with multiple 5'UTR coding for HepG2 GGT was obtained by RNase protection. Computer analysis of this 5'UTR detected the existence of a stable stem and loop structure containing multiple steroid modulatory elements.

引用

页码：88 / 92

页数：5

共 33 条

[1]

Ausubel FM., 1990, CURRENT PROTOCOLS MO

[2] TISSUE-STAGE-SPECIFIC AND DEVELOPMENTAL-STAGE-SPECIFIC METHYLATION IN THE 2 KIDNEY PROMOTERS OF THE RAT GAMMA-GLUTAMYL TRANSPEPTIDASE GENE [J].