THE 5' UNTRANSLATED REGION OF THE HUMAN GAMMA-GLUTAMYL-TRANSFERASE MESSENGER-RNA CONTAINS A TISSUE-SPECIFIC ACTIVE TRANSLATIONAL ENHANCER

被引:21
作者
DIEDERICH, M
WELLMAN, M
VISVIKIS, A
PUGA, A
SIEST, G
机构
[1] CTR MED, CNRS, URA 597, 30 RUE LIONNOIS, F-54000 NANCY, FRANCE
[2] UNIV CINCINNATI, DEPT ENVIRONM HLTH, CINCINNATI, OH 45267 USA
来源
FEBS LETTERS | 1993年 / 332卷 / 1-2期
关键词
GAMMA-GLUTAMYL-TRANSFERASE; 5' UNTRANSLATED REGION; TRANSIENT EXPRESSION; RNASE PROTECTION ANALYSIS; TRANSLATIONAL REGULATION; SECONDARY STRUCTURE;
D O I
10.1016/0014-5793(93)80490-L
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report the functional and structural analysis of the 5' untranslated region (5'UTR) of human hepatoma HepG2 gamma-glutamyltransferase (GGT) mRNA. Transient expression of a hybrid GGT-luciferase gene in HepG2, MIA-Pa-Ca-2 and MG 63 cell lines shows that this 5'UTR acts as a tissue-specific translational enhancer. Evidence for transcripts with multiple 5'UTR coding for HepG2 GGT was obtained by RNase protection. Computer analysis of this 5'UTR detected the existence of a stable stem and loop structure containing multiple steroid modulatory elements.
引用
收藏
页码:88 / 92
页数:5
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