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HELIX-COIL TRANSITION OF THE SELF-COMPLEMENTARY DG-DG-DA-DA-DT-DT-DC-DC DUPLEX
被引:76
作者
:
PATEL, DJ
论文数:
0
引用数:
0
h-index:
0
机构:
Bell Laboratories, Murray Hill, New Jersey, 07974
PATEL, DJ
CANUEL, LL
论文数:
0
引用数:
0
h-index:
0
机构:
Bell Laboratories, Murray Hill, New Jersey, 07974
CANUEL, LL
机构
:
[1]
Bell Laboratories, Murray Hill, New Jersey, 07974
来源
:
EUROPEAN JOURNAL OF BIOCHEMISTRY
|
1979年
/ 96卷
/ 02期
关键词
:
D O I
:
10.1111/j.1432-1033.1979.tb13037.x
中图分类号
:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号
:
071010 ;
081704 ;
摘要
:
The helix‐coil transition of the octanucleotide self‐complementary duplex dG‐dG‐dA‐dA‐dT‐dT‐dC‐dC has been monitored at the Watson‐Crick protons, the base and sugar nonexchangeable protons and the backbone phosphates by high‐resolution nuclear magnetic resonance (NMR) spectroscopy. The melting transition of the octanucleotide monitored by ultraviolet absorbance spectroscopy is characterized by the thermodynamic parameters ΔH°=–216.7 kJ/mol and ΔS° (25°C) =–0.632 kJ mol−1 K−1 in 0.1 M NaCl, 10 mM phosphate solution. Correlation of the transition midpoint values monitored by the ultraviolet absorbance studies at strand concentrations below 0.2 mM and by NMR studies at 5.3 mM suggest that both methods are monitoring the octanucleotide duplex‐to‐strand transition. The NMR spectra of the Watson‐Crick ring NH protons of the octanucleotide duplex have been followed as a function of temperature. The resonance from the terminal dG · dC base pairs broadens out at room temperature while the resonances from the other base pairs broaden simultaneously with the onset of the melting transition. The nonexchangeable base and sugar H‐1′ protons are resolved in the duplex and strand states and shift as average peaks through the melting transition. The experimental shifts on duplex formation have been compared with calculated values based on ring‐current and atomic diamagnetic anisotropy contributions for a B‐DNA base‐pair‐overlap geometry in solution. Several nonexchangeable proton resonances broaden in the fast‐exchange region during the duplex‐to‐strand transition and the excess widths yield a duplex dissociation rate constant for the octanucleotide of 1.9 × 103 s−1 at 32 °C (fraction of duplex = 0.86) in 0.1 M NaCl, 10 mM phosphate buffer. The 31P resonances of the seven internucleotide phosphates are distributed over 0.6 ppm in the duplex state, shift downfield during the duplex‐to‐strand transition and undergo additional downfield shifts during the stacked‐to‐unstacked strand transition with increasing temperature. Copyright © 1979, Wiley Blackwell. All rights reserved
引用
收藏
页码:267 / 276
页数:10
相关论文
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[1]
REFINEMENT OF STRUCTURE OF B-DNA AND IMPLICATIONS FOR ANALYSIS OF X-RAY-DIFFRACTION DATA FROM FIBERS OF BIOPOLYMERS
[J].
ARNOTT, S
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0
引用数:
0
h-index:
0
机构:
PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
ARNOTT, S
;
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PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
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PMR OF SELF-COMPLEMENTARY OLIGORIBONUCLEOTIDE CPCPGPG
[J].
ARTER, DB
论文数:
0
引用数:
0
h-index:
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UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
ARTER, DB
;
WALKER, GC
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0
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0
h-index:
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UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
WALKER, GC
;
UHLENBECK, OC
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UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
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UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
SCHMIDT, PG
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BORER, PN
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JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
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;
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0
h-index:
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JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
KAN, LS
;
TSO, POP
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引用数:
0
h-index:
0
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JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
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BORER PN, 1975, BIOCHEMISTRY-US, V14, P4864
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PROTON MAGNETIC RESONANCE STUDY OF SINGLE-STRANDED AND DOUBLE-HELICAL DEOXYRIBOOLIGONUCLEOTIDES
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CROSS, AD
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CROTHERS, DM
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NUCLEAR MAGNETIC-RESONANCE STUDY OF HYDROGEN-BONDED RING PROTONS IN WATSON-CRICK BASE PAIRS
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CROTHERS, DM
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0
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YALE UNIV,NEW HAVEN,CT 06520
CROTHERS, DM
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HILBERS, CW
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[J].
EARLY, TA
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0
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;
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UNIV CALIF SAN DIEGO,REVELLE COLL DEPT CHEM,LA JOLLA,CA 92037
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;
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引用数:
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论文数:
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论文数:
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引用数:
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BELL TEL LABS,MURRAY HILL,NJ 07974
BELL TEL LABS,MURRAY HILL,NJ 07974
PATEL, DJ
.
BIOCHEMISTRY,
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←
1
2
3
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共 31 条
[1]
REFINEMENT OF STRUCTURE OF B-DNA AND IMPLICATIONS FOR ANALYSIS OF X-RAY-DIFFRACTION DATA FROM FIBERS OF BIOPOLYMERS
[J].
ARNOTT, S
论文数:
0
引用数:
0
h-index:
0
机构:
PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
ARNOTT, S
;
HUKINS, DWL
论文数:
0
引用数:
0
h-index:
0
机构:
PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
HUKINS, DWL
.
JOURNAL OF MOLECULAR BIOLOGY,
1973,
81
(02)
:93
-105
[2]
PMR OF SELF-COMPLEMENTARY OLIGORIBONUCLEOTIDE CPCPGPG
[J].
ARTER, DB
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
ARTER, DB
;
WALKER, GC
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
WALKER, GC
;
UHLENBECK, OC
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UHLENBECK, OC
;
SCHMIDT, PG
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
UNIV ILLINOIS,SCH CHEM SCI,URBANA,IL 61801
SCHMIDT, PG
.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,
1974,
61
(04)
:1089
-1094
[3]
CONFORMATION AND INTERACTION OF SHORT NUCLEIC-ACID DOUBLE-STRANDED HELICES .1. PROTON MAGNETIC-RESONANCE STUDIES ON NONEXCHANGEABLE PROTONS OF RIBOSYL APAPGPCPUPU
[J].
BORER, PN
论文数:
0
引用数:
0
h-index:
0
机构:
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
BORER, PN
;
KAN, LS
论文数:
0
引用数:
0
h-index:
0
机构:
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
KAN, LS
;
TSO, POP
论文数:
0
引用数:
0
h-index:
0
机构:
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DIV BIOPHYS,BALTIMORE,MD 21205
TSO, POP
.
BIOCHEMISTRY,
1975,
14
(22)
:4847
-4863
[4]
BORER PN, 1975, BIOCHEMISTRY-US, V14, P4864
[5]
PROTON MAGNETIC RESONANCE STUDY OF SINGLE-STRANDED AND DOUBLE-HELICAL DEOXYRIBOOLIGONUCLEOTIDES
[J].
CROSS, AD
论文数:
0
引用数:
0
h-index:
0
CROSS, AD
;
CROTHERS, DM
论文数:
0
引用数:
0
h-index:
0
CROTHERS, DM
.
BIOCHEMISTRY,
1971,
10
(22)
:4015
-&
[6]
NUCLEAR MAGNETIC-RESONANCE STUDY OF HYDROGEN-BONDED RING PROTONS IN WATSON-CRICK BASE PAIRS
[J].
CROTHERS, DM
论文数:
0
引用数:
0
h-index:
0
机构:
YALE UNIV,NEW HAVEN,CT 06520
CROTHERS, DM
;
HILBERS, CW
论文数:
0
引用数:
0
h-index:
0
机构:
YALE UNIV,NEW HAVEN,CT 06520
HILBERS, CW
;
SHULMAN, RG
论文数:
0
引用数:
0
h-index:
0
机构:
YALE UNIV,NEW HAVEN,CT 06520
SHULMAN, RG
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1973,
70
(10)
:2899
-2901
[7]
HIGH-RESOLUTION PROTON NUCLEAR MAGNETIC-RESONANCE INVESTIGATION OF STRUCTURAL AND DYNAMIC PROPERTIES OF D(C15A15).D(T15G15)
[J].
EARLY, TA
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN DIEGO,REVELLE COLL DEPT CHEM,LA JOLLA,CA 92037
EARLY, TA
;
KEARNS, DR
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN DIEGO,REVELLE COLL DEPT CHEM,LA JOLLA,CA 92037
KEARNS, DR
;
BURD, JF
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN DIEGO,REVELLE COLL DEPT CHEM,LA JOLLA,CA 92037
BURD, JF
;
LARSON, JE
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN DIEGO,REVELLE COLL DEPT CHEM,LA JOLLA,CA 92037
LARSON, JE
;
WELLS, RD
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN DIEGO,REVELLE COLL DEPT CHEM,LA JOLLA,CA 92037
WELLS, RD
.
BIOCHEMISTRY,
1977,
16
(03)
:541
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[8]
ATOMIC OR LOCAL CONTRIBUTIONS TO PROTON CHEMICAL-SHIFTS DUE TO ANISOTROPY OF DIAMAGNETIC SUSCEPTIBILITY OF NUCLEIC-ACID BASES
[J].
GIESSNERPRETTRE, C
论文数:
0
引用数:
0
h-index:
0
机构:
INST BIOL PHYS CHIM,F-75005 PARIS,FRANCE
INST BIOL PHYS CHIM,F-75005 PARIS,FRANCE
GIESSNERPRETTRE, C
;
PULLMAN, B
论文数:
0
引用数:
0
h-index:
0
机构:
INST BIOL PHYS CHIM,F-75005 PARIS,FRANCE
INST BIOL PHYS CHIM,F-75005 PARIS,FRANCE
PULLMAN, B
.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,
1976,
70
(02)
:578
-581
[9]
INTERACTION OF MIRACIL D WITH DOUBLE-STRANDED POLY(ADENYLIC ACID) POLY(URIDYLIC ACID)
[J].
HELLER, MJ
论文数:
0
引用数:
0
h-index:
0
机构:
COLORADO STATE UNIV,DEPT CHEM,FT COLLINS,CO 80521
HELLER, MJ
;
TU, AT
论文数:
0
引用数:
0
h-index:
0
机构:
COLORADO STATE UNIV,DEPT CHEM,FT COLLINS,CO 80521
TU, AT
;
MACIEL, GE
论文数:
0
引用数:
0
h-index:
0
机构:
COLORADO STATE UNIV,DEPT CHEM,FT COLLINS,CO 80521
MACIEL, GE
.
BIOCHEMISTRY,
1974,
13
(08)
:1623
-1631
[10]
PROTON NUCLEAR MAGNETIC-RESONANCE INVESTIGATIONS OF NUCLEATION AND PROPAGATION REACTIONS ASSOCIATED WITH HELIX-COIL TRANSITION OF D-APTPGPCPAPT IN H2O SOLUTION
[J].
HILBERS, CW
论文数:
0
引用数:
0
h-index:
0
机构:
BELL TEL LABS,MURRAY HILL,NJ 07974
BELL TEL LABS,MURRAY HILL,NJ 07974
HILBERS, CW
;
PATEL, DJ
论文数:
0
引用数:
0
h-index:
0
机构:
BELL TEL LABS,MURRAY HILL,NJ 07974
BELL TEL LABS,MURRAY HILL,NJ 07974
PATEL, DJ
.
BIOCHEMISTRY,
1975,
14
(12)
:2656
-2660
←
1
2
3
4
→