CONTINUOUS PRODUCTION OF NADP BY IMMOBILIZED BREVIBACTERIUM-AMMONIAGENES CELLS

被引：25

作者：

MURATA, K

KATO, J

CHIBATA, I

机构：

[1] Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co, Ltd, Yodogawa-ku, Osaka

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 05期

关键词：

D O I：

10.1002/bit.260210511

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whole cells of Brevibacterium ammoniagenes IAM 1645 having the polyphosphate NAD‐kinase were successfully immobilized in a polyacrylamide gel lattice. The immobilized cells were activated by treatment with organic solvents or detergents. The pH optimum of the immobilized cells for the production of NADP was 7.0, and divalent metal ions were required to maintain the elevated activity of polyphosphate NAD‐kinase. Highly pure NADP was continuously produced in high yield by the immobilized cell column. The half‐life of this column was about eight days. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：887 / 895

页数：9