THE PAR REGION OF PSC101 AFFECTS PLASMID COPY NUMBER AS WELL AS STABILITY

被引：33

作者：

MANEN, D ^{[1
]}

GOEBEL, T ^{[1
]}

CARO, L ^{[1
]}

机构：

[1] UNIV GENEVA,DEPT MOLEC BIOL,30 QUAI ANSERMET,CH-1211 GENEVA 4,SWITZERLAND

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 11期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb02032.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The par locus is a segment of pSC101 that has been identified as a cis‐acting determinant of plasmid stability. We show that par also determines copy number and must, therefore, play a role in plasmid replication. The segregation defect, but not the copy‐number reduction, of par‐ replication origins is completely suppressed by a short sequence from the bacteriophage lambda gene O which is present in plasmid pKO‐4. Thus, replication and segregation functions are separable from each other. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1839 / 1846

页数：8

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