Changes of intracellular calcium activity (Cai(2+)) in mouse mammary epithelial cells in primary culture (normal) and in an established cell line (MMT060562, cancerous) were investigated by microfluorometry and image analysis of fura-2 fluorescence. In both types of cells, some populations exhibited occasional Cai(2+) oscillations with a period of 50 - 160 s. Slight mechanical stimulation of a cell with a fine glass pipette induced a Cai(2+) increase, which spread from the stimulated cell to the surrounding cells with a speed of 7-12 mum/s. ATP (> 1 mumol/1) and ADP. but not AMP induced a Cai(2+) increase in both cell types. Bradykinin was highly effective (> 10 nmol/l) only in the cancerous mammary epithelial cells. In Ca2+-free solution, all these Cai(2+) responses remained unchanged at the first application, and decreased abruptly at the second trial. La3+ (> 0.5 mmol/1) suppressed the response to ATP but not the response to bradykinin. Addition of extracellular Mn2+ rapidly quenched the fura-2 fluorescence in the cell even in a non-stimulated state. Influx of Mn2+ did not increase during Cai(2+) responses. These results indicate that the sources of Cai(2+) responses in mammary epithelial cells are intracellular stores, which exchange Ca2+ with the extracellular medium.
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL
DOSREIS, GA
PERSECHINI, PM
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL
PERSECHINI, PM
RIBEIRO, JMC
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL
RIBEIRO, JMC
OLIVEIRACASTRO, GM
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL
DOSREIS, GA
PERSECHINI, PM
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL
PERSECHINI, PM
RIBEIRO, JMC
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL
RIBEIRO, JMC
OLIVEIRACASTRO, GM
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UNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZILUNIV FED RIO DE JANEIRO, CTR CIENCIAS SAUDE, ILHA FDN, INST BIOFIS, RIO DE JANEIRO, RJ, BRAZIL